Difference between revisions of "SkfA"
| Line 14: | Line 14: | ||
|style="background:#ABCDEF;" align="center"|'''Function''' || killing of sister cells | |style="background:#ABCDEF;" align="center"|'''Function''' || killing of sister cells | ||
|- | |- | ||
| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU01910 skfA] |
|- | |- | ||
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/SkfA SkfA] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/SkfA SkfA] | ||
| Line 24: | Line 24: | ||
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ybcM]]'', ''[[skfB]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ybcM]]'', ''[[skfB]]'' | ||
|- | |- | ||
| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU01910 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU01910 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU01910 Advanced_DNA] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:ybcO_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:ybcO_context.gif]] | ||
Revision as of 12:01, 13 May 2013
- Description: spore killing factor
| Gene name | skfA |
| Synonyms | ybcO |
| Essential | no |
| Product | spore killing factor |
| Function | killing of sister cells |
| Gene expression levels in SubtiExpress: skfA | |
| Interactions involving this protein in SubtInteract: SkfA | |
| MW, pI | 5 kDa, 10.648 |
| Gene length, protein length | 165 bp, 55 aa |
| Immediate neighbours | ybcM, skfB |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
toxins, antitoxins and immunity against toxins
This gene is a member of the following regulons
AbrB regulon, PhoP regulon, Spo0A regulon
The gene
Basic information
- Locus tag: BSU01910
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification: the mature SKF is cyclic 26-amino acid peptide that is posttranslationally modified with one disulfide and one cysteine thioether bridged to the alpha-position of a methionine PubMed, the thioether bond is generated by SkfB PubMed
- Cofactor(s):
- Effectors of protein activity:
- Localization: secreted (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: O31422
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
- Northern blotting during during phosphate limitation showed an intense 0.25 kb skfA-specific transcript, and a weaker 6.5 kb skfA-skfB-skfC-skfE-skfF-skfG-skfH transcript.
- The skfA transcript is stabilized by a stem-loop structure in the skfA-skfB intergenic region. PubMed
- The mRNA is very stable (half-life > 15 min) PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
José Eduardo González-Pastor
Cannibalism: a social behavior in sporulating Bacillus subtilis.
FEMS Microbiol Rev: 2011, 35(3);415-24
[PubMed:20955377]
[WorldCat.org]
[DOI]
(I p)
Original Publications
Additional publications: PubMed
