Difference between revisions of "AlkA"

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|style="background:#ABCDEF;" align="center"|'''Function''' || adaptive response to alkylative DNA damage
 
|style="background:#ABCDEF;" align="center"|'''Function''' || adaptive response to alkylative DNA damage
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU01800 alkA]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU01800 alkA]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 35 kDa, 8.147   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 35 kDa, 8.147   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[glmS]]'', ''[[adaA]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[glmS]]'', ''[[adaA]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB11956&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU01800 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU01800 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU01800 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:alkA_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:alkA_context.gif]]

Revision as of 12:00, 13 May 2013

  • Description: DNA-3-methyladenine glycosylase

Gene name alkA
Synonyms
Essential no
Product DNA-3-methyladenine glycosylase
Function adaptive response to alkylative DNA damage
Gene expression levels in SubtiExpress: alkA
MW, pI 35 kDa, 8.147
Gene length, protein length 909 bp, 303 aa
Immediate neighbours glmS, adaA
Sequences Protein DNA Advanced_DNA
Genetic context
AlkA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
AlkA expression.png















Categories containing this gene/protein

DNA repair/ recombination

This gene is a member of the following regulons

AdaA regulon

The gene

Basic information

  • Locus tag: BSU01800

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine (according to Swiss-Prot)
  • Protein family: alkylbase DNA glycosidase alkA family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism: AdaA: positive regulation PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

F Morohoshi, K Hayashi, N Munkata
Bacillus subtilis alkA gene encoding inducible 3-methyladenine DNA glycosylase is adjacent to the ada operon.
J Bacteriol: 1993, 175(18);6010-7
[PubMed:8376346] [WorldCat.org] [DOI] (P p)