Difference between revisions of "YacF"

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|style="background:#ABCDEF;" align="center"|'''Function''' || tRNA maturation
 
|style="background:#ABCDEF;" align="center"|'''Function''' || tRNA maturation
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU00810 yacF]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00810 yacF]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/folate_biosynthesis.html Folate]'''
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/folate_biosynthesis.html Folate]'''
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yazB]]'', ''[[lysS]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yazB]]'', ''[[lysS]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB11857&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU00810 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU00810 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU00810 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:yacF_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yacF_context.gif]]

Revision as of 11:56, 13 May 2013

  • Description: putative tRNA-dihydrouridine synthase B

Gene name yacF
Synonyms dusB, dus1
Essential no
Product putative tRNA-dihydrouridine synthase B
Function tRNA maturation
Gene expression levels in SubtiExpress: yacF
Metabolic function and regulation of this protein in SubtiPathways:
Folate
MW, pI 36 kDa, 7.868
Gene length, protein length 999 bp, 333 aa
Immediate neighbours yazB, lysS
Sequences Protein DNA Advanced_DNA
Genetic context
YacF context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YacF expression.png




























Categories containing this gene/protein

translation

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU00810

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: dus family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1VHN (from Thermotoga maritima, 35% identity, 54% similarity) PubMed
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information: the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References