Difference between revisions of "BacG"
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=== Basic information/ Evolution === | === Basic information/ Evolution === | ||
| − | * '''Catalyzed reaction/ biological activity:''' YwfH catalyzes the conjugate addition of hydride at the C4 olefinic terminus using NADH to yield the cyclohexenol- containing tetrahydro-4-hydroxyphenylpyruvate {{PubMed|20052993}} | + | * '''Catalyzed reaction/ biological activity:''' |
| + | ** YwfH catalyzes the conjugate addition of hydride at the C4 olefinic terminus using NADH to yield the cyclohexenol- containing tetrahydro-4-hydroxyphenylpyruvate {{PubMed|20052993}} | ||
| + | ** stereoselective reduction of dihydro-hydroxyphenylpyruvate (H2HPP) to tetrahydro-hydroxyphenylpyruvate (H4HPP), NADPH-dependent reductase that facilitates the conjugate addition of a hydride at the C(4) olefin terminus of H2HPP {{PubMed|23519407}} | ||
* '''Protein family:''' short-chain dehydrogenases/reductases (SDR) family (according to Swiss-Prot) | * '''Protein family:''' short-chain dehydrogenases/reductases (SDR) family (according to Swiss-Prot) | ||
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* '''Modification:''' | * '''Modification:''' | ||
| − | * '''Cofactor(s):''' | + | * '''Cofactor(s):''' NADPH |
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
| − | * '''Interactions:''' | + | * '''[[SubtInteract|Interactions]]:''' |
| − | * '''Localization:''' | + | * '''[[Localization]]:''' |
=== Database entries === | === Database entries === | ||
| − | * '''Structure:''' | + | * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=3u49 3U49] {{PubMed|23519407}} |
* '''UniProt:''' [http://www.uniprot.org/uniprot/P39644 P39644] | * '''UniProt:''' [http://www.uniprot.org/uniprot/P39644 P39644] | ||
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ywfH_3867493_3868272_1 ywfH] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ywfH_3867493_3868272_1 ywfH] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' | + | * '''[[Sigma factor]]:''' |
* '''Regulation:''' | * '''Regulation:''' | ||
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=References= | =References= | ||
| − | <pubmed> 12372825 12107147 20052993 21948839</pubmed> | + | <pubmed> 12372825 12107147 20052993 21948839 23519407 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 14:32, 27 March 2013
- Description: short chain reductase involved in bacilysin synthesis
| Gene name | ywfH |
| Synonyms | ipa-86r |
| Essential | no |
| Product | short chain reductase |
| Function | biosynthesis of the antibiotic bacilysin |
| Gene expression levels in SubtiExpress: ywfH | |
| MW, pI | 27 kDa, 6.596 |
| Gene length, protein length | 777 bp, 259 aa |
| Immediate neighbours | ywfI, bacF |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
miscellaneous metabolic pathways, biosynthesis of antibacterial compounds
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU37680
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- YwfH catalyzes the conjugate addition of hydride at the C4 olefinic terminus using NADH to yield the cyclohexenol- containing tetrahydro-4-hydroxyphenylpyruvate PubMed
- stereoselective reduction of dihydro-hydroxyphenylpyruvate (H2HPP) to tetrahydro-hydroxyphenylpyruvate (H4HPP), NADPH-dependent reductase that facilitates the conjugate addition of a hydride at the C(4) olefin terminus of H2HPP PubMed
- Protein family: short-chain dehydrogenases/reductases (SDR) family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s): NADPH
- Effectors of protein activity:
Database entries
- UniProt: P39644
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon: ywfH PubMed
- Regulation:
- repressed by casamino acids PubMed
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Malligarjunan Rajavel, Kumar Perinbam, B Gopal
Structural insights into the role of Bacillus subtilis YwfH (BacG) in tetrahydrotyrosine synthesis.
Acta Crystallogr D Biol Crystallogr: 2013, 69(Pt 3);324-32
[PubMed:23519407]
[WorldCat.org]
[DOI]
(I p)
Takashi Inaoka, Kozo Ochi
Scandium stimulates the production of amylase and bacilysin in Bacillus subtilis.
Appl Environ Microbiol: 2011, 77(22);8181-3
[PubMed:21948839]
[WorldCat.org]
[DOI]
(I p)
Sarah A Mahlstedt, Christopher T Walsh
Investigation of anticapsin biosynthesis reveals a four-enzyme pathway to tetrahydrotyrosine in Bacillus subtilis.
Biochemistry: 2010, 49(5);912-23
[PubMed:20052993]
[WorldCat.org]
[DOI]
(I p)
Takashi Inaoka, Kosaku Takahashi, Mayumi Ohnishi-Kameyama, Mitsuru Yoshida, Kozo Ochi
Guanine nucleotides guanosine 5'-diphosphate 3'-diphosphate and GTP co-operatively regulate the production of an antibiotic bacilysin in Bacillus subtilis.
J Biol Chem: 2003, 278(4);2169-76
[PubMed:12372825]
[WorldCat.org]
[DOI]
(P p)
Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147]
[WorldCat.org]
[DOI]
(P p)
