Difference between revisions of "Era"

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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 903 bp, 301 aa  
 
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 903 bp, 301 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[recO]]'', ''[[cdd]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yqzL]]'', ''[[cdd]]''
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14458&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14458&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''

Revision as of 13:54, 3 December 2012

  • Description: GTP-binding protein, involved in the assembly of the 30S ribosomal subunit

Gene name era
Synonyms bex, yqfH
Essential yes PubMed
Product GTP-binding protein
Function ribosome assembly
Gene expression levels in SubtiExpress: era
MW, pI 33 kDa, 8.376
Gene length, protein length 903 bp, 301 aa
Immediate neighbours yqzL, cdd
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Era context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Era expression.png




























Categories containing this gene/protein

translation, essential genes, GTP-binding proteins

This gene is a member of the following regulons

SigB regulon

The gene

Basic information

  • Locus tag: BSU25290

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Binds and hydrolyzes GTP and readily exchanges GDP for GTP
  • Protein family: Era subfamily (according to Swiss-Prot) Era/Obg family

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
    • for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP837, available in Stülke lab
    • for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP845, available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Naotake Ogasawara, Nara, Japan

Your additional remarks

References

Reviews

Natalie Verstraeten, Maarten Fauvart, Wim Versées, Jan Michiels
The universally conserved prokaryotic GTPases.
Microbiol Mol Biol Rev: 2011, 75(3);507-42, second and third pages of table of contents
[PubMed:21885683] [WorldCat.org] [DOI] (I p)

Robert A Britton
Role of GTPases in bacterial ribosome assembly.
Annu Rev Microbiol: 2009, 63;155-76
[PubMed:19575570] [WorldCat.org] [DOI] (I p)


Original publications