FlhF

• Description: signal recognition particle-like GTPase, placement and assembly of flagella, control of basal body position
  
  

Gene name	flhF
Synonyms
Essential	no
Product	signal recognition particle-like GTPase
Function	placement and assembly of polar flagella
Gene expression levels in SubtiExpress: flhF
Interactions involving this protein in SubtInteract: FlhF
MW, pI	40 kDa, 8.719
Gene length, protein length	1098 bp, 366 aa
Immediate neighbours	flhA, flhG
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

motility and chemotaxis, membrane proteins

This gene is a member of the following regulons

CodY regulon, SigD regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU16400

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • GTPase activity PubMed
  • placement of the first flagellar protein, FliF PubMed

• Protein family: GTP-binding SRP family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:
  • interaction with FlhG activates the GTPase activity of FlhF PubMed

• Interactions:
  • FlhG-FlhF (FlhG binds the GTP-bound homodimer of FlhF) PubMed

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• Structure:
  • 2PX3 (complexed with GTP-Mg2+) PubMed
  • 3SYN (complex with its activator YlxH) PubMed

• UniProt: Q01960

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-ylxH-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed
  • ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-ylxH-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed

• Expression browser: flhF PubMed

• Sigma factor:
  • flgB: SigA PubMed, SigD PubMed
  • ylxF: SigD PubMed

• Regulation:
  • repressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed
  • CodY: transcription repression PubMed
  • DegU (non-phosphorylated): transcription activation PubMed

• Additional information:

Biological materials

• Mutant:
  • 1A925 ( flhF::cat), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: