FrlM

• Description: aminosugar ABC transporter (permease)
  
  

Gene name	frlM
Synonyms	yurM
Essential	no
Product	aminosugar ABC transporter (permease)
Function	uptake of sugar amines
Gene expression levels in SubtiExpress: frlM
Interactions involving this protein in SubtInteract: FrlM
Metabolic function and regulation of this protein in SubtiPathways: Alternative nitrogen sources
MW, pI	34 kDa, 7.114
Gene length, protein length	900 bp, 300 aa
Immediate neighbours	frlD, frlN
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

ABC transporters, utilization of specific carbon sources, utilization of nitrogen sources other than amino acids, membrane proteins

This gene is a member of the following regulons

CodY regulon, FrlR regulon

The gene

Basic information

• Locus tag: BSU32580

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: MalFG subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • YurJ-(FrlM-FrlN)-FrlO PubMed

• Localization: cell membrane PubMed

Database entries

• Structure:

• UniProt: O32154

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:
  • yurR-yurQ-frlB-frlO-frlN-frlM-frlD PubMed
  • frlB-frlO-frlN-frlM-frlD PubMed

• Expression browser: frlM PubMed

• Sigma factor:

• Regulation:
  • frlB: repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • frlB: induced in the presence of fructosamine (FrlR) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed1, PubMed2
  • FrlR: transcription repression PubMed

• Additional information:
  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed
  • the frlB-frlO-frlN-frlM-frlD operon is not expressed in a cshA mutant PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional references: PubMed

Martin Lehnik-Habrink, Leonie Rempeters, Ákos T Kovács, Christoph Wrede, Claudia Baierlein, Heike Krebber, Oscar P Kuipers, Jörg Stülke
DEAD-Box RNA helicases in Bacillus subtilis have multiple functions and act independently from each other.
J Bacteriol: 2013, 195(3);534-44
[PubMed:23175651] [WorldCat.org] [DOI] (I p)

Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J  
RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y. 
Mol Microbiol. 2011 81(6): 1459-1473. 
PubMed:21815947