Difference between revisions of "YkuI"

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(Biological materials)
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=Biological materials =
 
=Biological materials =
  
* '''Mutant:'''
+
* '''Mutant:''' GP1324 (tet) available in [[Stülke]] lab
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 11:37, 30 October 2012

  • Description: potential c-di-GMP degrading phosphodiesterase

Gene name ykuI
Synonyms
Essential no
Product potential c-di-GMP degrading phosphodiesterase
Function unknown
Gene expression levels in SubtiExpress: ykuI
MW, pI 47 kDa, 4.462
Gene length, protein length 1221 bp, 407 aa
Immediate neighbours ykuH, fsrA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YkuI context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YkuI expression.png
























Categories containing this gene/protein

metabolism of signalling nucleotides

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU14090

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: degradation of cyclic di-GMP PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains: EAL domain (aa 1 ... 245, for c-di-GMP hydrolysis), PAS-like domain (aa 300 ... 400) PubMed
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP1324 (tet) available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed