TsaB

• Description: cofactor of the Gcp tRNA modification enzyme
  
  

Gene name	ydiC
Synonyms
Essential	yes PubMed
Product	cofactor of the Gcp tRNA modification enzyme
Function	tRNA modification
Gene expression levels in SubtiExpress: ydiC
Interactions involving this protein in SubtInteract: YdiC
MW, pI	25 kDa, 5.762
Gene length, protein length	687 bp, 229 aa
Immediate neighbours	ydiB, ydiD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

essential genes, translation

The gene

Basic information

• Locus tag: BSU05920

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • biosynthesis of the threonylcarbamoyladenosine (t6A) residue at position 37 of ANN-decoding tRNAs using L-threonylcarbamoyl-AMP (TsaB-TsaD-TsaE) PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • TsaB-TsaD PubMed
  • TsaB-TsaE PubMed

• Localization:

Database entries

• Structure:

• UniProt: O05516

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: ydiC PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Charles T Lauhon
Mechanism of N6-threonylcarbamoyladenonsine (t(6)A) biosynthesis: isolation and characterization of the intermediate threonylcarbamoyl-AMP.
Biochemistry: 2012, 51(44);8950-63
[PubMed:23072323] [WorldCat.org] [DOI] (I p)

Basma El Yacoubi, Isabelle Hatin, Christopher Deutsch, Tamer Kahveci, Jean-Pierre Rousset, Dirk Iwata-Reuyl, Alexey G Murzin, Valérie de Crécy-Lagard
A role for the universal Kae1/Qri7/YgjD (COG0533) family in tRNA modification.
EMBO J: 2011, 30(5);882-93
[PubMed:21285948] [WorldCat.org] [DOI] (I p)

Jennifer I Handford, Bérengère Ize, Grant Buchanan, Gareth P Butland, Jack Greenblatt, Andrew Emili, Tracy Palmer
Conserved network of proteins essential for bacterial viability.
J Bacteriol: 2009, 191(15);4732-49
[PubMed:19376873] [WorldCat.org] [DOI] (I p)

Alison Hunt, Joy P Rawlins, Helena B Thomaides, Jeff Errington
Functional analysis of 11 putative essential genes in Bacillus subtilis.
Microbiology (Reading): 2006, 152(Pt 10);2895-2907
[PubMed:17005971] [WorldCat.org] [DOI] (P p)