Difference between revisions of "TsaC"

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(Expression and regulation)
(References)
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[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 08:09, 18 October 2012

  • Description: L-threonylcarbamoyl-AMP synthase, biosynthesis of the hypermodified base threonylcarbamoyladenosine (t(6)A) (together with TsaB-TsaD-TsaE)

Gene name ywlC
Synonyms ipc-29d
Essential no
Product L-threonylcarbamoyl-AMP synthase
Function tRNA modification
Gene expression levels in SubtiExpress: tsaC
MW, pI 36 kDa, 5.82
Gene length, protein length 1038 bp, 346 aa
Immediate neighbours ywlD, ywlB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YwlC context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YwlC expression.png















Categories containing this gene/protein

translation

This gene is a member of the following regulons

DnaA regulon

The gene

Basic information

  • Locus tag: BSU36950

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • conversion of L-threonine, bicarbonate/CO2 and ATP to give the intermediate L-threonylcarbamoyl-AMP (TC-AMP) and pyrophosphate as products PubMed
    • biosynthesis of the hypermodified base threonylcarbamoyladenosine (t(6)A), a universal modification found at position 37 of ANN decoding tRNAs, which imparts a unique structure to the anticodon loop enhancing its binding to ribosomes in vitro (shown for the corresponding E. coli protein YrdC) PubMed
  • Protein family: SUA5 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1HRU (the homolog from E. coli) PubMed
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References