TcyP

• Description: cystine transporter, membrane protein
  
  

Gene name	tcyP
Synonyms	yhcL
Essential	no
Product	cystine transporter, membrane protein
Function	cystine uptake
Gene expression levels in SubtiExpress: tcyP
Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate
MW, pI	48 kDa, 9.471
Gene length, protein length	1389 bp, 463 aa
Immediate neighbours	yhcK, yhcM
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, biosynthesis/ acquisition of amino acids, membrane proteins

This gene is a member of the following regulons

CymR regulon

The gene

Basic information

• Locus tag: BSU09130

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: cystine-proton symporter PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information: K(m) = 0.6 myM PubMed

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • membrane associated PubMed

Database entries

• Structure:

• UniProt: P54596

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: tcyP

• Expression browser: tcyP PubMed

• Sigma factor:

• Regulation:
  • repressed in the presence of sulfate or cysteine (CymR) PubMed

• Regulatory mechanism:
  • CymR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • 1A949 ( tcyP::spec), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Isabelle Martin-Verstraete, Institute Pasteur, Paris, France