Difference between revisions of "List of plasmids"
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*[[pGP574]]: expression of proteins in ''E. coli'' allows fusion to a Strep-tag at the C-terminus of the protein | *[[pGP574]]: expression of proteins in ''E. coli'' allows fusion to a Strep-tag at the C-terminus of the protein | ||
| − | == Construction of (markerless) deletions | + | == Construction of (markerless) deletions == |
| − | *[[pMAD]]: | + | *[[pMAD]]: construction of markerless KOs in Gram-positive bacteria |
Revision as of 09:28, 30 August 2012
Here you can find a list of Bacillus subtilis plasmids:
Contents
Fusion to reporter genes
- pAC5: translational lacZ-fusions (chloramphenicol resistence)
- pAC6: transcriptional lacZ-fusions (cat resistence)
- pAC7: translational lacZ-fusions (kanamycin resistence)
- pBaSysBioII: gfp transcriptional fusions for high-throughput analysis of gene expression PubMed
- a suite of vectors for ectopic insertion of GFP, CFP and IYFP transcriptional fusions in single copy at the amyE and bglS loci PubMed
Labelling of a protein with a flourescent tag
- pGP1080: integrative plasmid for the fusion of GFP tag to the C-terminus of a protein, keeping the control of the expression under its natural promoter, allows express of the downstream gene by the P(spac) promoter
- pGP1870: integrative plasmid for the fusion of GFP tag to the C-terminus of a protein, keeping the control of the expression under its natural promoter
- pGP1871: integrative plasmid for the fusion of YFP tag to the C-terminus of a protein, keeping the control of the expression under its natural promoter
Labelling of a protein with a triple FLAG tag
- pGP1331: integrative plasmid for the fusion of 3x FLAG tag to the C-terminus of a protein, keeping expression under the control of the natural promoter
- pGP1087: integrative plasmid for the fusion of 3x FLAG tag to the C-terminus of a protein, keeping expression under the control of the natural promoter, allowing the expression of downstream genes under the control of the IPTG-inducible Pspac promoter
Expression/ purification in B. subtilis
- pBQ200: overexpression of proteins in B. subtilis under control of a strong degQ36 promoter
- pGP380: expression of proteins in B. subtilis allows fusion to a Strep-tag at the N-terminus of the protein
- pGP382: expression of proteins in B. subtilis allows fusion to a Strep-tag at the C-terminus of the protein
- pGP888: integration vector (integrates in ganA); allowing the expression of genes under the control of the xylose-inducible PxylA promoter in B. subtilis
- pGP1459: integration vector (integrates in ganA) analogous to pGP380; expression of proteins in B. subtilis allows fusion to a Strep-tag at the N-terminus of the protein
- pGP1460: integration vector (integrates in ganA) analogous to pGP382; expression of proteins in B. subtilis allows fusion to a Strep-tag at the C-terminus of the protein
- pGP1389: integrative plasmid for the fusion of a Strep-tag to the C-terminus of a protein, keeping expression under the control of the natural promoter
Expression/ purification in E. coli
- pWH844: expression of proteins in E. coli, allows fusion to a N-terminal His-tag
- pGP570: expression of proteins in E. coli, allows fusion to a N-terminal His-tag that can be cleaved off
- pGP172: expression of proteins in E. coli allows fusion to a Strep-tag at the N-terminus of the protein
- pGP574: expression of proteins in E. coli allows fusion to a Strep-tag at the C-terminus of the protein
Construction of (markerless) deletions
- pMAD: construction of markerless KOs in Gram-positive bacteria
