Difference between revisions of "WprA"

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= This gene is a member of the following [[regulons]] =
 
= This gene is a member of the following [[regulons]] =
{{SubtiWiki regulon|[[YvrHb regulon]]}}
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{{SubtiWiki regulon|[[YvrHb regulon]]}},
 +
{{SubtiWiki regulon|[[CcpA regulon]]}}
  
 
=The gene=
 
=The gene=
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=wprA_1153789_1156473_1 wprA] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=wprA_1153789_1156473_1 wprA] {{PubMed|22383849}}
  
* '''Sigma factor:'''  
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* '''Sigma factor:''' [[SigA]] {{PubMed|22900538}}
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
 +
** repressed by glucose ([[CcpA]]) {{PubMed|22900538}}
  
* '''Regulatory mechanism:''' activated by [[YvrHb]] [http://www.ncbi.nlm.nih.gov/sites/entrez/16306698 PubMed]
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* '''Regulatory mechanism:'''  
 +
** [[CcpA]]: transcription repression {{PubMed|22900538}}
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** [[YvrHb]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/16306698 PubMed]
  
 
* '''Additional information:'''
 
* '''Additional information:'''
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=References=
 
=References=
 +
<pubmed> 22900538</pubmed>
 
  <big>''Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J''  </big>
 
  <big>''Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J''  </big>
 
  <big>'''RNA processing in ''Bacillus subtilis'': identification of targets of the essential RNase Y.''' </big>
 
  <big>'''RNA processing in ''Bacillus subtilis'': identification of targets of the essential RNase Y.''' </big>

Revision as of 16:49, 23 August 2012

  • Description: cell wall-associated protein precursor

Gene name wprA
Synonyms yisM
Essential no
Product cell wall-associated protein precursor
Function unknown
Gene expression levels in SubtiExpress: wprA
MW, pI 96 kDa, 9.58
Gene length, protein length 2682 bp, 894 aa
Immediate neighbours yisL, yisN
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
WprA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
WprA expression.png















Categories containing this gene/protein

cell wall/ other

This gene is a member of the following regulons

YvrHb regulon, CcpA regulon

The gene

Basic information

  • Locus tag: BSU10770

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: peptidase S8 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J  
RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y. 
Mol Microbiol. 2011 81(6): 1459-1473. 
PubMed:21815947

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Masakuni Serizawa, Keisuke Kodama, Hiroki Yamamoto, Kazuo Kobayashi, Naotake Ogasawara, Junichi Sekiguchi
Functional analysis of the YvrGHb two-component system of Bacillus subtilis: identification of the regulated genes by DNA microarray and northern blot analyses.
Biosci Biotechnol Biochem: 2005, 69(11);2155-69
[PubMed:16306698] [WorldCat.org] [DOI] (P p)

K Stephenson, C L Jensen, S T Jørgensen, C R Harwood
Simultaneous inactivation of the wprA and dltB genes of Bacillus subtilis reduces the yield of alpha-amylase.
Lett Appl Microbiol: 2002, 34(6);394-7
[PubMed:12028417] [WorldCat.org] [DOI] (P p)

Haike Antelmann, Hiroki Yamamoto, Junichi Sekiguchi, Michael Hecker
Stabilization of cell wall proteins in Bacillus subtilis: a proteomic approach.
Proteomics: 2002, 2(5);591-602
[PubMed:11987133] [WorldCat.org] [DOI] (P p)

J H Hageman, R L Switzer
ISP-4 and CWBP52 are proteins encoded by the same gene in Bacillus subtilis.
Microbiology (Reading): 1999, 145 ( Pt 2);281
[PubMed:10075409] [WorldCat.org] [DOI] (P p)

K Stephenson, C R Harwood
Influence of a cell-wall-associated protease on production of alpha-amylase by Bacillus subtilis.
Appl Environ Microbiol: 1998, 64(8);2875-81
[PubMed:9687444] [WorldCat.org] [DOI] (P p)

P Margot, D Karamata
The wprA gene of Bacillus subtilis 168, expressed during exponential growth, encodes a cell-wall-associated protease.
Microbiology (Reading): 1996, 142 ( Pt 12);3437-44
[PubMed:9004506] [WorldCat.org] [DOI] (P p)