Difference between revisions of "CycB"

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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1263 bp, 421 aa  
 
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1263 bp, 421 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yvfL]]'', ''[[lacR]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ganP]]'', ''[[lacR]]''
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15421&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15421&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 +
* a ''[[cycB]]-[[ganP]]-[[ganQ]]-[[ganA]]-[[ganB]]'' mutant is impaired in the utilization of galactan {{PubMed|22893383}}
  
 
=== Database entries ===
 
=== Database entries ===
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=== Additional information===
 
=== Additional information===
 
 
  
  
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* '''[[SubtInteract|Interactions]]:'''
 
* '''[[SubtInteract|Interactions]]:'''
** ([[YvfL]]-[[YvfM]])-[[CycB]] {{PubMed|10092453}}
+
** ([[GanP]]-[[GanQ]])-[[CycB]] {{PubMed|10092453}}
  
 
* '''[[Localization]]:''' cell membrane (via [[YvfL]]-[[YvfM]]) {{PubMed|10092453}}
 
* '''[[Localization]]:''' cell membrane (via [[YvfL]]-[[YvfM]]) {{PubMed|10092453}}
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=Expression and regulation=
 
=Expression and regulation=
* '''Operon:''' ''[[cycB]]-[[yvfL]]-[[yvfM]]-[[lacA]]-[[yvfO]]'' {{PubMed|9287030}}  
+
* '''Operon:''' ''[[cycB]]-[[ganP]]-[[ganQ]]-[[ganA]]-[[ganB]]'' {{PubMed|9287030}}  
  
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cycB_3507253_3508518_-1 cycB] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cycB_3507253_3508518_-1 cycB] {{PubMed|22383849}}

Revision as of 13:41, 16 August 2012

Gene name cycB
Synonyms yvfK
Essential no
Product cyclodextrin/maltodextrin ABC transporter, binding protein
Function maltodextrin utilization
Gene expression levels in SubtiExpress: cycB
Interactions involving this protein in SubtInteract: CycB
MW, pI 45 kDa, 5.441
Gene length, protein length 1263 bp, 421 aa
Immediate neighbours ganP, lacR
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YvfK context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CycB expression.png















Categories containing this gene/protein

ABC transporters, utilization of specific carbon sources, membrane proteins

This gene is a member of the following regulons

LacR regulon

The gene

Basic information

  • Locus tag: BSU34160

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: bacterial solute-binding protein 1 family (according to Swiss-Prot)
  • Paralogous protein(s): MdxE

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

A Kamionka, M K Dahl
Bacillus subtilis contains a cyclodextrin-binding protein which is part of a putative ABC-transporter.
FEMS Microbiol Lett: 2001, 204(1);55-60
[PubMed:11682178] [WorldCat.org] [DOI] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)

R A Daniel, J Haiech, F Denizot, J Errington
Isolation and characterization of the lacA gene encoding beta-galactosidase in Bacillus subtilis and a regulator gene, lacR.
J Bacteriol: 1997, 179(17);5636-8
[PubMed:9287030] [WorldCat.org] [DOI] (P p)