GabT

• Description: gamma-aminobutyrate transaminase, general stress protein
  
  

Gene name	gabT
Synonyms	ycnG
Essential	no
Product	gamma-aminobutyrate transaminase
Function	utilization of gamma-amino butyric acid and synthesis of norspermidine
Gene expression levels in SubtiExpress: gabT
MW, pI	47 kDa, 5.304
Gene length, protein length	1308 bp, 436 aa
Immediate neighbours	gabR, gabD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of amino acids, miscellaneous metabolic pathways, biofilm formation

This gene is a member of the following regulons

GabR regulon

The gene

Basic information

• Locus tag: BSU03900

Phenotypes of a mutant

• • block in norspermidine production PubMed
  • impaired biofilm disassembly PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • 4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate (according to Swiss-Prot)

• Protein family: class-III pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P94427

• KEGG entry: [3]

• E.C. number: 2.6.1.19

Expression and regulation

• Operon: gabT-gabD PubMed

• Expression browser: gabT PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed in the presence of gamma-butyric acid (GabR) PubMed

• Regulatory mechanism:
  • GabR: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ilana Kolodkin-Gal, Shugeng Cao, Liraz Chai, Thomas Böttcher, Roberto Kolter, Jon Clardy, Richard Losick
A self-produced trigger for biofilm disassembly that targets exopolysaccharide.
Cell: 2012, 149(3);684-92
[PubMed:22541437] [WorldCat.org] [DOI] (I p)

Boris R Belitsky
Bacillus subtilis GabR, a protein with DNA-binding and aminotransferase domains, is a PLP-dependent transcriptional regulator.
J Mol Biol: 2004, 340(4);655-64
[PubMed:15223311] [WorldCat.org] [DOI] (P p)

Boris R Belitsky, Abraham L Sonenshein
GabR, a member of a novel protein family, regulates the utilization of gamma-aminobutyrate in Bacillus subtilis.
Mol Microbiol: 2002, 45(2);569-83
[PubMed:12123465] [WorldCat.org] [DOI] (P p)