CotR

• Description: spore protein, lipolytic enzyme
  
  

Gene name	cotR
Synonyms	yvdO
Essential	no
Product	spore lipolytic enzyme
Function	hydrolysis of lysophospholipids
Gene expression levels in SubtiExpress: cotR
MW, pI	35 kDa, 10.2
Gene length, protein length	960 bp, 320 aa
Immediate neighbours	cotQ, trnQ-Arg
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of lipids, sporulation proteins

This gene is a member of the following regulons

SigK regulon

The gene

Basic information

• Locus tag: BSU34530

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: release of myristic acid from 1-myristoly-2-lyso-sn-glycero-3-phosphocholine PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • spore wall (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O06996

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: cotR PubMed

• Sigma factor: SigK PubMed

• Regulation:
  • expressed late during sporulation in the mother cell (SigK) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Shiro Kato, Tohru Yoshimura, Hisashi Hemmi, Ryuichi Moriyama
Biochemical analysis of a novel lipolytic enzyme YvdO from Bacillus subtilis 168.
Biosci Biotechnol Biochem: 2010, 74(4);701-6
[PubMed:20378985] [WorldCat.org] [DOI] (I p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

S Little, A Driks
Functional analysis of the Bacillus subtilis morphogenetic spore coat protein CotE.
Mol Microbiol: 2001, 42(4);1107-20
[PubMed:11737650] [WorldCat.org] [DOI] (P p)