Difference between revisions of "NasE"

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* '''Description:''' assimilatory nitrite reductase (subunit) <br/><br/>
  
 
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|style="background:#ABCDEF;" align="center"|'''Function''' || utilization of nitrite as nitrogen source  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || utilization of nitrite as nitrogen source  
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU03290 nasE]
 
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/NasE NasE]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/NasE NasE]
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|colspan="2" | '''Genetic context''' <br/> [[Image:nasE_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:nasE_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=nasE_355412_355732_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:nasE_expression.png|500px]]
 
 
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* '''Operon:''' ''[[nasB]]-[[nasC]]-[[nasD]]-[[nasE]]-[[nasF]]'' {{PubMed|7836289}}  
 
* '''Operon:''' ''[[nasB]]-[[nasC]]-[[nasD]]-[[nasE]]-[[nasF]]'' {{PubMed|7836289}}  
  
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=nasE_355412_355732_-1 nasE] {{PubMed|22383849}}
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* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|7836289}}
 
 
* '''Sigma factor:''' [[SigA]] {{PubMed|7836289}}
 
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  

Revision as of 11:05, 7 August 2012

  • Description: assimilatory nitrite reductase (subunit)

Gene name nasE
Synonyms nasBD, nirD
Essential no
Product assimilatory nitrite reductase (subunit)
Function utilization of nitrite as nitrogen source
Interactions involving this protein in SubtInteract: NasE
Metabolic function and regulation of this protein in SubtiPathways:
Alternative nitrogen sources
MW, pI 11 kDa, 4.805
Gene length, protein length 318 bp, 106 aa
Immediate neighbours nasF, nasD
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
NasE context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

respiration, utilization of nitrogen sources other than amino acids

This gene is a member of the following regulons

Fur regulon, NsrR regulon, ResD regulon, TnrA regulon

The gene

Basic information

  • Locus tag: BSU03290

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Ammonium hydroxide + 3 NAD(P)+ + H2O = nitrite + 3 NAD(P)H (according to Swiss-Prot)
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
    • expressed under anaerobic conditions (ResD) PubMed
    • induced by nitric oxide under anaerobic conditions (NsrR) PubMed
    • induced by iron starvation (Fur) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Víctor M Luque-Almagro, Andrew J Gates, Conrado Moreno-Vivián, Stuart J Ferguson, David J Richardson, M Dolores Roldán
Bacterial nitrate assimilation: gene distribution and regulation.
Biochem Soc Trans: 2011, 39(6);1838-43
[PubMed:22103536] [WorldCat.org] [DOI] (I p)

Original publications