Difference between revisions of "YcbF"

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* '''Description:''' glucarate dehydratase <br/><br/>
  
 
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|style="background:#ABCDEF;" align="center"|'''Function''' || glucarate utilization
 
|style="background:#ABCDEF;" align="center"|'''Function''' || glucarate utilization
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU02490 ycbF]
 
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 50 kDa, 5.594   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 50 kDa, 5.594   
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|colspan="2" | '''Genetic context''' <br/> [[Image:ycbF_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:ycbF_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gudD_271800_273167_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:ycbF_expression.png|500px]]
 
 
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* '''Operon:''' ''[[ycbC]]-[[ycbD]]-[[ycbE]]-[[ycbF]]-[[ycbG]]-[[ycbH]]-[[ycbJ]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/12044674 PubMed]
 
* '''Operon:''' ''[[ycbC]]-[[ycbD]]-[[ycbE]]-[[ycbF]]-[[ycbG]]-[[ycbH]]-[[ycbJ]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/12044674 PubMed]
  
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gudD_271800_273167_1 ycbF] {{PubMed|22383849}}
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* '''[[Sigma factor]]:'''  
 
 
* '''Sigma factor:'''  
 
  
 
* '''Regulation:''' induced by D-glucarate/galactarate ([[YcbG]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12044674 PubMed]
 
* '''Regulation:''' induced by D-glucarate/galactarate ([[YcbG]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12044674 PubMed]

Revision as of 10:46, 7 August 2012

  • Description: glucarate dehydratase

Gene name ycbF
Synonyms gudD
Essential no
Product glucarate dehydratase
Function glucarate utilization
MW, pI 50 kDa, 5.594
Gene length, protein length 1365 bp, 455 aa
Immediate neighbours ycbE, ycbG
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YcbF context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

YcbG regulon

The gene

Basic information

  • Locus tag: BSU02490

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: d-glucarate = 5-dehydro-4-deoxy-D-glucarate + H2O (according to Swiss-Prot)
  • Protein family: GlucD subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation: induced by D-glucarate/galactarate (YcbG) PubMed
  • Regulatory mechanism: YcbG: transcription repression PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Shigeo Hosoya, Kunio Yamane, Michio Takeuchi, Tsutomu Sato
Identification and characterization of the Bacillus subtilis D-glucarate/galactarate utilization operon ycbCDEFGHJ.
FEMS Microbiol Lett: 2002, 210(2);193-9
[PubMed:12044674] [WorldCat.org] [DOI] (P p)