Difference between revisions of "AlkA"

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* '''Description:''' DNA-3-methyladenine glycosylase <br/><br/>
  
 
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|style="background:#ABCDEF;" align="center"|'''Function''' || adaptive response to alkylative DNA damage
 
|style="background:#ABCDEF;" align="center"|'''Function''' || adaptive response to alkylative DNA damage
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU01800 alkA]
 
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 35 kDa, 8.147   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 35 kDa, 8.147   
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|colspan="2" | '''Genetic context''' <br/> [[Image:alkA_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:alkA_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=alkA_202547_203458_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:alkA_expression.png|500px]]
 
 
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* '''Operon:''' [[alkA]] {{PubMed|8376346}}  
 
* '''Operon:''' [[alkA]] {{PubMed|8376346}}  
  
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=alkA_202547_203458_-1 alkA] {{PubMed|22383849}}
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* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|8376346}}
 
 
* '''Sigma factor:''' [[SigA]] {{PubMed|8376346}}
 
 
   
 
   
 
* '''Regulation:'''  
 
* '''Regulation:'''  

Revision as of 10:40, 7 August 2012

  • Description: DNA-3-methyladenine glycosylase

Gene name alkA
Synonyms
Essential no
Product DNA-3-methyladenine glycosylase
Function adaptive response to alkylative DNA damage
MW, pI 35 kDa, 8.147
Gene length, protein length 909 bp, 303 aa
Immediate neighbours ybbU, adaA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
AlkA context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

DNA repair/ recombination

This gene is a member of the following regulons

AdaA regulon

The gene

Basic information

  • Locus tag: BSU01800

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine (according to Swiss-Prot)
  • Protein family: alkylbase DNA glycosidase alkA family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism: AdaA: positive regulation PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

F Morohoshi, K Hayashi, N Munkata
Bacillus subtilis alkA gene encoding inducible 3-methyladenine DNA glycosylase is adjacent to the ada operon.
J Bacteriol: 1993, 175(18);6010-7
[PubMed:8376346] [WorldCat.org] [DOI] (P p)