TilS

Gene name	tilS
Synonyms	yacA
Essential	yes PubMed
Product	tRNAIle-lysidine synthetase
Function	tRNA modification
Gene expression levels in SubtiExpress: tilS
Metabolic function and regulation of this protein in SubtiPathways: Nucleotides (regulation)
MW, pI	53 kDa, 8.317
Gene length, protein length	1416 bp, 472 aa
Immediate neighbours	yabT, hprT
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, cell envelope stress proteins (controlled by SigM, V, W, X, Y), essential genes

This gene is a member of the following regulons

SigM regulon

The gene

Basic information

• Locus tag: BSU00670

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: tRNA(Ile)-lysidine synthase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • Cytoplasm (Homogeneous) PubMed

Database entries

• Structure: 3A2K (from Geobacillus kaustophilus, complex with tRNA) PubMed

• UniProt: P37563

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: tilS-hprT-ftsH PubMed

• Expression browser: tilS PubMed

• Sigma factor: SigM PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Original publications

Céline Fabret, Etienne Dervyn, Bérengère Dalmais, Alain Guillot, Christian Marck, Henri Grosjean, Philippe Noirot
Life without the essential bacterial tRNA Ile2-lysidine synthetase TilS: a case of tRNA gene recruitment in Bacillus subtilis.
Mol Microbiol: 2011, 80(4);1062-74
[PubMed:21435031] [WorldCat.org] [DOI] (I p)

Kotaro Nakanishi, Luc Bonnefond, Satoshi Kimura, Tsutomu Suzuki, Ryuichiro Ishitani, Osamu Nureki
Structural basis for translational fidelity ensured by transfer RNA lysidine synthetase.
Nature: 2009, 461(7267);1144-8
[PubMed:19847269] [WorldCat.org] [DOI] (I p)

Scott P Salowe, Judyann Wiltsie, Julio C Hawkins, Lisa M Sonatore
The catalytic flexibility of tRNAIle-lysidine synthetase can generate alternative tRNA substrates for isoleucyl-tRNA synthetase.
J Biol Chem: 2009, 284(15);9656-62
[PubMed:19233850] [WorldCat.org] [DOI] (P p)

Warawan Eiamphungporn, John D Helmann
The Bacillus subtilis sigma(M) regulon and its contribution to cell envelope stress responses.
Mol Microbiol: 2008, 67(4);830-48
[PubMed:18179421] [WorldCat.org] [DOI] (P p)

Alison Hunt, Joy P Rawlins, Helena B Thomaides, Jeff Errington
Functional analysis of 11 putative essential genes in Bacillus subtilis.
Microbiology (Reading): 2006, 152(Pt 10);2895-2907
[PubMed:17005971] [WorldCat.org] [DOI] (P p)

Jean-Christophe Meile, Ling Juan Wu, S Dusko Ehrlich, Jeff Errington, Philippe Noirot
Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory.
Proteomics: 2006, 6(7);2135-46
[PubMed:16479537] [WorldCat.org] [DOI] (P p)

Akiko Soma, Yoshiho Ikeuchi, Satoru Kanemasa, Kazuo Kobayashi, Naotake Ogasawara, Tomotake Ote, Jun-ichi Kato, Kimitsuna Watanabe, Yasuhiko Sekine, Tsutomu Suzuki
An RNA-modifying enzyme that governs both the codon and amino acid specificities of isoleucine tRNA.
Mol Cell: 2003, 12(3);689-98
[PubMed:14527414] [WorldCat.org] [DOI] (P p)