Difference between revisions of "NifZ"
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| − | * '''Description:''' cysteine desulfurase<br/><br/> | + | * '''Description:''' cysteine desulfurase, biosynthesis of 4-thiouridine in tRNA<br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
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|style="background:#ABCDEF;" align="center"| '''Product''' || cysteine desulfurase | |style="background:#ABCDEF;" align="center"| '''Product''' || cysteine desulfurase | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"|'''Function''' || | + | |style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of 4-thiouridine in tRNA |
| + | |- | ||
| + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/NifZ NifZ] | ||
|- | |- | ||
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/thiamin.html Thiamin]''' | |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/thiamin.html Thiamin]''' | ||
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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1143 bp, 381 aa | |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1143 bp, 381 aa | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ | + | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[thiI]]'', ''[[braB]]'' |
|- | |- | ||
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/nifZ_nucleotide.txt Gene sequence (+200bp) ]''' | |style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/nifZ_nucleotide.txt Gene sequence (+200bp) ]''' | ||
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<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
| − | {{SubtiWiki category|[[ | + | {{SubtiWiki category|[[translation]]}} |
= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
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=== Basic information/ Evolution === | === Basic information/ Evolution === | ||
| − | * '''Catalyzed reaction/ biological activity:''' | + | * '''Catalyzed reaction/ biological activity:''' removes sulfuryl group from cysteine and transfers it to [[ThiI]] {{PubMed|22773787}} |
* '''Protein family:''' | * '''Protein family:''' | ||
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* '''[[SubtInteract|Interactions]]:''' | * '''[[SubtInteract|Interactions]]:''' | ||
| + | ** [[NifZ]]-[[ThiI]] {{PubMed|22773787}} | ||
* '''[[Localization]]:''' | * '''[[Localization]]:''' | ||
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<pubmed>19348578 </pubmed> | <pubmed>19348578 </pubmed> | ||
==Original publications== | ==Original publications== | ||
| − | <pubmed>10715213</pubmed> | + | <pubmed>10715213 22773787</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 09:29, 10 July 2012
- Description: cysteine desulfurase, biosynthesis of 4-thiouridine in tRNA
| Gene name | nifZ |
| Synonyms | iscSB |
| Essential | no |
| Product | cysteine desulfurase |
| Function | biosynthesis of 4-thiouridine in tRNA |
| Interactions involving this protein in SubtInteract: NifZ | |
| Metabolic function and regulation of this protein in SubtiPathways: Thiamin | |
| MW, pI | 41 kDa, 6.34 |
| Gene length, protein length | 1143 bp, 381 aa |
| Immediate neighbours | thiI, braB |
| Gene sequence (+200bp) | Protein sequence |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU29590
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: removes sulfuryl group from cysteine and transfers it to ThiI PubMed
- Protein family:
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- UniProt: O34874
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Christopher T Jurgenson, Tadhg P Begley, Steven E Ealick
The structural and biochemical foundations of thiamin biosynthesis.
Annu Rev Biochem: 2009, 78;569-603
[PubMed:19348578]
[WorldCat.org]
[DOI]
(I p)
Original publications
Lauren J Rajakovich, John Tomlinson, Patricia C Dos Santos
Functional Analysis of Bacillus subtilis Genes Involved in the Biosynthesis of 4-Thiouridine in tRNA.
J Bacteriol: 2012, 194(18);4933-40
[PubMed:22773787]
[WorldCat.org]
[DOI]
(I p)
J T Kaiser, T Clausen, G P Bourenkow, H D Bartunik, S Steinbacher, R Huber
Crystal structure of a NifS-like protein from Thermotoga maritima: implications for iron sulphur cluster assembly.
J Mol Biol: 2000, 297(2);451-64
[PubMed:10715213]
[WorldCat.org]
[DOI]
(P p)
