Difference between revisions of "GabT"

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|style="background:#ABCDEF;" align="center"| '''Product''' || gamma-aminobutyrate transaminase
 
|style="background:#ABCDEF;" align="center"| '''Product''' || gamma-aminobutyrate transaminase
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || utilization of gamma-amino butyric acid  
+
|style="background:#ABCDEF;" align="center"|'''Function''' || utilization of gamma-amino butyric acid and synthesis of norspermidine
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 47 kDa, 5.304   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 47 kDa, 5.304   
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= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[utilization of amino acids]]}}
+
{{SubtiWiki category|[[utilization of amino acids]]}},
 +
{{SubtiWiki category|[[miscellaneous metabolic pathways]]}},
 +
{{SubtiWiki category|[[biofilm formation]]}}
  
 
= This gene is a member of the following [[regulons]] =
 
= This gene is a member of the following [[regulons]] =
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 +
** block in norspermidine production {{PubMed|22541437}}
 +
** impaired biofilm disassembly  {{PubMed|22541437}}
  
 
=== Database entries ===
 
=== Database entries ===
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=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
* '''Catalyzed reaction/ biological activity:''' 4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate (according to Swiss-Prot)  
+
* '''Catalyzed reaction/ biological activity:'''  
 +
** 4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate (according to Swiss-Prot)  
  
 
* '''Protein family:''' class-III pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)
 
* '''Protein family:''' class-III pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)
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=References=
 
=References=
  
<pubmed>15223311,12123465,, </pubmed>
+
<pubmed>15223311,12123465,22541437, </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 15:09, 6 May 2012

  • Description: gamma-aminobutyrate transaminase, general stress protein

Gene name gabT
Synonyms ycnG
Essential no
Product gamma-aminobutyrate transaminase
Function utilization of gamma-amino butyric acid and synthesis of norspermidine
MW, pI 47 kDa, 5.304
Gene length, protein length 1308 bp, 436 aa
Immediate neighbours gabR, gabD
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
GabT context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
GabT expression.png
























Categories containing this gene/protein

utilization of amino acids, miscellaneous metabolic pathways, biofilm formation

This gene is a member of the following regulons

GabR regulon

The gene

Basic information

  • Locus tag: BSU03900

Phenotypes of a mutant

    • block in norspermidine production PubMed
    • impaired biofilm disassembly PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • 4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate (according to Swiss-Prot)
  • Protein family: class-III pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expressed in the presence of gamma-butyric acid (GabR) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ilana Kolodkin-Gal, Shugeng Cao, Liraz Chai, Thomas Böttcher, Roberto Kolter, Jon Clardy, Richard Losick
A self-produced trigger for biofilm disassembly that targets exopolysaccharide.
Cell: 2012, 149(3);684-92
[PubMed:22541437] [WorldCat.org] [DOI] (I p)

Boris R Belitsky
Bacillus subtilis GabR, a protein with DNA-binding and aminotransferase domains, is a PLP-dependent transcriptional regulator.
J Mol Biol: 2004, 340(4);655-64
[PubMed:15223311] [WorldCat.org] [DOI] (P p)

Boris R Belitsky, Abraham L Sonenshein
GabR, a member of a novel protein family, regulates the utilization of gamma-aminobutyrate in Bacillus subtilis.
Mol Microbiol: 2002, 45(2);569-83
[PubMed:12123465] [WorldCat.org] [DOI] (P p)