Difference between revisions of "ThrZ"

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|colspan="2" | '''Genetic context''' <br/> [[Image:thrZ_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:thrZ_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
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|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=thrZ_3854256_3856172_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:thrZ_expression.png|500px]]
 
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Revision as of 11:28, 23 April 2012

  • Description: threonyl-tRNA synthetase (minor)

Gene name thrZ
Synonyms thrS2
Essential no
Product threonyl-tRNA synthetase (minor)
Function translation
Metabolic function and regulation of this protein in SubtiPathways:
tRNA charging
MW, pI 73 kDa, 5.753
Gene length, protein length 1914 bp, 638 aa
Immediate neighbours ywhA, mmr
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
ThrZ context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
ThrZ expression.png




























Categories containing this gene/protein

translation

This gene is a member of the following regulons

T-box

The gene

Basic information

  • Locus tag: BSU37560

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr) (according to Swiss-Prot)
  • Protein family: class-II aminoacyl-tRNA synthetase family (according to Swiss-Prot)
  • Paralogous protein(s): ThrS, one of the two proteins has to be present for viability PubMed

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1TJE (from Escherichia coli, 43% identity, 65% similarity) PubMed
  • KEGG entry: [3]

Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

  • Regulation:
  • Additional information:
    • subject to Clp-dependent proteolysis upon glucose starvation PubMed
    • the amount of the mRNA is substantially decreased upon depletion of RNase Y PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J  
RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y. 
Mol Microbiol. 2011 81(6): 1459-1473. 
PubMed:21815947