Difference between revisions of "SucD"
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|colspan="2" | '''Genetic context''' <br/> [[Image:sucD_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:sucD_context.gif]] | ||
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div> | <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div> | ||
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+ | |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sucD_1681617_1682519_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:sucD_expression.png|500px]] | ||
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Revision as of 09:32, 19 April 2012
- Description: succinyl-CoA synthetase (alpha subunit)
Gene name | sucD |
Synonyms | |
Essential | no |
Product | succinyl-CoA synthetase (alpha subunit) |
Function | TCA cycle |
Interactions involving this protein in SubtInteract: SucD | |
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism | |
MW, pI | 31 kDa, 5.587 |
Gene length, protein length | 900 bp, 300 aa |
Immediate neighbours | sucC, dprA |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
ATP synthesis, carbon core metabolism, phosphoproteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU16100
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + succinate + CoA = ADP + phosphate + succinyl-CoA (according to Swiss-Prot)
- Protein family: succinate/malate CoA ligase alpha subunit family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information: Reversible Michaelis-Menten FEBS Letters
- Domains:
- Modification: phosphorylation on (Ser-19 OR Thr-20) PubMed
- Cofactor(s):
- Effectors of protein activity:
- Inhibited by 2-oxoglutarate, ATP and NADH FEBS Letters
- GTP is not accepted by the enzyme FEBS Letters
Database entries
- Structure: 1JKJ (E. coli)
- UniProt: P80865
- KEGG entry: [3]
- E.C. number: 6.2.1.5
Additional information
- extensive information on the structure and enzymatic properties of succinyl-CoA synthetase can be found at Proteopedia
Expression and regulation
- Sigma factor:
- Additional information:
Biological materials
- Mutant: GP720 (spc), available in Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
References