Difference between revisions of "GltB"

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* '''Locus tag:''' BSU18440
 
* '''Locus tag:''' BSU18440
 
[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gltB_2008572_2010053_-1 Expression]
 
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===

Revision as of 09:59, 28 January 2012

  • Description: small subunit of glutamate synthase

Gene name gltB
Synonyms
Essential no
Product glutamate synthase (small subunit)
Function glutamate biosynthesis
Interactions involving this protein in SubtInteract: GltB
Metabolic function and regulation of this protein in SubtiPathways:
Ammonium/ glutamate
MW, pI 54.6 kDa, 7.69
Gene length, protein length 1479 bp, 493 amino acids
Immediate neighbours yogA, gltA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
GltB context.gif
This image was kindly provided by SubtiList








Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

GltC regulon, TnrA regulon

The gene

Basic information

  • Locus tag: BSU18440

Phenotypes of a mutant

auxotrophic for glutamate

Database entries

  • DBTBS entry: [1]
  • SubtiList entry:[2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH (according to Swiss-Prot) 2 L-glutamate + NADP(+) <=> L-glutamine + 2-oxoglutarate + NADPH
  • Protein family: glutamate synthase family.
  • Paralogous protein(s): none

Extended information on the protein

  • Kinetic information:
  • Domains:
    • nucleotide binding domain (NADP) (299–313)
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation: see gltA
  • Additional information:

Biological materials

  • Mutant: GP807 (del gltAB::tet), GP517 (ermC), both available in Stülke lab
  • Expression vector:
    • pGP1119 (in pGP380, for SPINE, expression in B. subtilis), available in Stülke lab
  • lacZ fusion: see gltA
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • Antibody:

Labs working on this gene/protein

Linc Sonenshein, Tufts University, Boston, MA, USA Homepage

Jörg Stülke, University of Göttingen, Germany Homepage

Your additional remarks

References

Reviews

Original publications