Difference between revisions of "MiaA"
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* '''Locus tag:''' BSU17330 | * '''Locus tag:''' BSU17330 | ||
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===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
Revision as of 09:41, 28 January 2012
- Description: tRNA isopentenylpyrophosphate transferase
| Gene name | miaA |
| Synonyms | |
| Essential | no |
| Product | tRNA isopentenylpyrophosphate transferase |
| Function | tRNA modification |
| MW, pI | 35 kDa, 6.002 |
| Gene length, protein length | 942 bp, 314 aa |
| Immediate neighbours | ymaF, hfq |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU17330
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
- A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Isopentenyl diphosphate + tRNA = diphosphate + tRNA containing 6-isopentenyladenosine (according to Swiss-Prot)
- Protein family: IPP transferase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: O31795
- KEGG entry: [2]
- E.C. number: 2.5.1.8
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
The corresponding gene/ protein in other bacteria
J Zhao, H E Leung, M E Winkler
The miaA mutator phenotype of Escherichia coli K-12 requires recombination functions.
J Bacteriol: 2001, 183(5);1796-800
[PubMed:11160115]
[WorldCat.org]
[DOI]
(P p)
J M Durand, G R Björk, A Kuwae, M Yoshikawa, C Sasakawa
The modified nucleoside 2-methylthio-N6-isopentenyladenosine in tRNA of Shigella flexneri is required for expression of virulence genes.
J Bacteriol: 1997, 179(18);5777-82
[PubMed:9294434]
[WorldCat.org]
[DOI]
(P p)
J Caillet, L Droogmans
Molecular cloning of the Escherichia coli miaA gene involved in the formation of delta 2-isopentenyl adenosine in tRNA.
J Bacteriol: 1988, 170(9);4147-52
[PubMed:3045085]
[WorldCat.org]
[DOI]
(P p)
