GlpD

• Description: glycerol-3-phosphate dehydrogenase (menaquinone 7)
  
  

Gene name	glpD
Synonyms
Essential	no
Product	glycerol-3-phosphate dehydrogenase (menaquinone 7)
Function	glycerol utilization
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	62 kDa, 8.002
Gene length, protein length	1665 bp, 555 aa
Immediate neighbours	glpK, pgcA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

utilization of specific carbon sources, utilization of lipids

This gene is a member of the following regulons

AbrB regulon, CcpA regulon, GlpP regulon

The gene

Basic information

• Locus tag: BSU09300

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: sn-glycerol 3-phosphate + a quinone = glycerone phosphate + a quinol (according to Swiss-Prot)

• Protein family: FAD-dependent glycerol-3-phosphate dehydrogenase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): FAD

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 3DA1 (from Bacillus halodurans, 57% identity, 72% similarity)

• UniProt: P18158

• KEGG entry: [3]

• E.C. number: 1.1.5.3

Expression and regulation

• Operon: glpD PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by glucose (CcpA)
  • induced by glycerol (GlpP) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression
  • GlpP: transcriptional antitermination at a RNA switch PubMed
  • AbrB: transcription activation PubMed

• Additional information:

Biological materials

• Mutant: QB5347 (cat), available in the Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed