Difference between revisions of "TapA"

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(Expression and regulation)
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* '''Locus tag:''' BSU24640
 
* '''Locus tag:''' BSU24640
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[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yqxM_2554486_2555247_-1 Expression]
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===

Revision as of 13:33, 25 January 2012

  • Description: required for the anchoring of the TasA amyloid fibers to the cell and for the initiation of fiber polymerization, minor fiber component



Gene name tapA
Synonyms yqhD, yqxM
Essential no
Product TasA anchoring/assembly protein
Function biofilm formation
Interactions involving this protein in SubtInteract: TapA
Regulation of this protein in SubtiPathways:
Biofilm, Protein secretion
MW, pI 28 kDa, 6.677
Gene length, protein length 759 bp, 253 aa
Immediate neighbours sipW, yqzG
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YqxM context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

biofilm formation, membrane proteins

This gene is a member of the following regulons

AbrB regulon, SinR regulon

The gene

Basic information

  • Locus tag: BSU24640

Expression

Phenotypes of a mutant

The mutants are able to form a biofilm in the presence of D-amino acids PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity: D-amino acids lead to disappearance of TapA from the cell wall PubMed
  • Localization:
    • attached to the cell surface (on the outside of the cell), associated with peptidoglycan PubMed
    • secretion requires SipW

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion: pGP1926 (in pAC6), available in Stülke lab
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original publications

Diethmaier C, Pietack N, Gunka K, Wrede C, Lehnik-Habrink M, Herzberg C, Hübner S, Stülke J  
A Novel Factor Controlling Bistability in Bacillus subtilis: The YmdB Protein Affects
Flagellin Expression and Biofilm Formation. 
J Bacteriol.: 2011, 193(21):5997-6007. 
PubMed:21856853
Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J  
RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y. 
Mol Microbiol. 2011 81(6): 1459-1473. 
PubMed:21815947