NdoA

• Description: RNase EndoA, MazF family toxin, UACAU-specific mRNA interferase
  
  

Gene name	ndoA
Synonyms	ydcE
Essential	no
Product	mRNA interferase
Function	unknown
Interactions involving this protein in SubtInteract: NdoA
MW, pI	12 kDa, 4.892
Gene length, protein length	348 bp, 116 aa
Immediate neighbours	ndoAI, rsbR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

Rnases, toxins, antitoxins and immunity against toxins

The gene

Basic information

• Locus tag: BSU04660

Expression

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: specifically cleaves a five-base sequence, UACAU PubMed

• Protein family: pemK family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity: the activity is inhibited by the interaction with NdoAI PubMed

• Interactions:
  • NdoAI-NdoA PubMed

• Localization:

Database entries

• Structure: 1NE8

• UniProt: P96622

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ndoAI-ndoA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • An antisense RNA is predicted for ndoA PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Xiangli Wu, Xiuhong Wang, Karl Drlica, Xilin Zhao
A toxin-antitoxin module in Bacillus subtilis can both mitigate and amplify effects of lethal stress.
PLoS One: 2011, 6(8);e23909
[PubMed:21897862] [WorldCat.org] [DOI] (I p)

Jung-Ho Park, Yoshihiro Yamaguchi, Masayori Inouye
Bacillus subtilis MazF-bs (EndoA) is a UACAU-specific mRNA interferase.
FEBS Lett: 2011, 585(15);2526-32
[PubMed:21763692] [WorldCat.org] [DOI] (I p)

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

Shivangi Agarwal, Shivani Agarwal, Rakesh Bhatnagar
Identification and characterization of a novel toxin-antitoxin module from Bacillus anthracis.
FEBS Lett: 2007, 581(9);1727-34
[PubMed:17416361] [WorldCat.org] [DOI] (P p)

Olivier Pellegrini, Nathalie Mathy, Arhonda Gogos, Lawrence Shapiro, Ciarán Condon
The Bacillus subtilis ydcDE operon encodes an endoribonuclease of the MazF/PemK family and its inhibitor.
Mol Microbiol: 2005, 56(5);1139-48
[PubMed:15882409] [WorldCat.org] [DOI] (P p)