YcbF

• Description: glucarate dehydratase
  
  

Gene name	ycbF
Synonyms	gudD
Essential	no
Product	glucarate dehydratase
Function	glucarate utilization
MW, pI	50 kDa, 5.594
Gene length, protein length	1365 bp, 455 aa
Immediate neighbours	ycbE, ycbG
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

YcbG regulon

The gene

Basic information

• Locus tag: BSU02490

Expression

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: d-glucarate = 5-dehydro-4-deoxy-D-glucarate + H₂O (according to Swiss-Prot)

• Protein family: GlucD subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P42238

• KEGG entry: [3]

• E.C. number: 4.2.1.40

Expression and regulation

• Operon: ycbC-ycbD-ycbE-ycbF-ycbG-ycbH-ycbJ PubMed

• Sigma factor:

• Regulation: induced by D-glucarate/galactarate (YcbG) PubMed

• Regulatory mechanism: YcbG: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Shigeo Hosoya, Kunio Yamane, Michio Takeuchi, Tsutomu Sato
Identification and characterization of the Bacillus subtilis D-glucarate/galactarate utilization operon ycbCDEFGHJ.
FEMS Microbiol Lett: 2002, 210(2);193-9
[PubMed:12044674] [WorldCat.org] [DOI] (P p)