Difference between revisions of "HypR"

From SubtiWiki
Jump to: navigation, search
Line 1: Line 1:
* '''Description:''' MarR/DUF24-family transcription regulator, positively controls the nitroreductase gene ''[[hypO]]'' in response to disulfide stress <br/><br/>
+
* '''Description:''' MarR/DUF24-family transcription regulator, positively controls the nitroreductase gene ''[[yfkO]]'' (hypO) in response to disulfide stress <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
| ''hypR''  
+
| ''yybR'' (hypR)
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yybR''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''hypR''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
Line 12: Line 12:
 
|style="background:#ABCDEF;" align="center"| '''Product''' || MarR/DUF24-family transcription regulator HypR   
 
|style="background:#ABCDEF;" align="center"| '''Product''' || MarR/DUF24-family transcription regulator HypR   
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || positively controls nitroreductase gene ''[[hypO]]'' in response to disulfide stress (diamide, NaOCl)
+
|style="background:#ABCDEF;" align="center"|'''Function''' || positively controls nitroreductase gene yfkO (hypO) in response to disulfide stress (diamide, NaOCl)
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 14 kDa, 8.415   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 14 kDa, 8.415   
Line 38: Line 38:
  
 
= This gene is a member of the following [[regulons]] =
 
= This gene is a member of the following [[regulons]] =
{{SubtiWiki regulon|[[HypR regulon]]}}
+
{{SubtiWiki regulon|[[YybR (HypR) regulon]]}}
  
= The [[HypR regulon]]:=
+
= The [[YybR (HypR) regulon]]:=
* ''[[hypR]]'', ''[[hypO]]''  
+
* ''[[yybR]]'' (hypR), ''[[yfkO]]'' (hypO)
  
 
=The gene=
 
=The gene=
Line 58: Line 58:
  
 
=== Additional information===
 
=== Additional information===
* ''[[hypR]]'' is autoregulated by disulfide stress
+
hypR autoregulated by disulfide stress
  
  
Line 69: Line 69:
 
* '''Protein family:''' MarR/DUF24-family regulator
 
* '''Protein family:''' MarR/DUF24-family regulator
  
* '''Paralogous protein(s):''' [[YdeP]], [[YkvN]]
+
* '''Paralogous protein(s):''' YdeP,YkvN
  
 
=== Extended information on the protein ===
 
=== Extended information on the protein ===

Revision as of 08:56, 13 January 2012

  • Description: MarR/DUF24-family transcription regulator, positively controls the nitroreductase gene yfkO (hypO) in response to disulfide stress

Gene name yybR (hypR)
Synonyms hypR
Essential no
Product MarR/DUF24-family transcription regulator HypR
Function positively controls nitroreductase gene yfkO (hypO) in response to disulfide stress (diamide, NaOCl)
MW, pI 14 kDa, 8.415
Gene length, protein length 375 bp, 125 aa
Immediate neighbours cotF, ppaC
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YybR context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

transcription factors and their control, resistance against oxidative and electrophile stress

This gene is a member of the following regulons

This gene is a member of the following regulons

YybR (HypR) regulon

The YybR (HypR) regulon:

The gene

Basic information

  • Locus tag: BSU40540

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

hypR autoregulated by disulfide stress


The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: MarR/DUF24-family regulator
  • Paralogous protein(s): YdeP,YkvN

Extended information on the protein

  • Kinetic information: Cys14 redox sensing Cys, has lower pKa of 6.36 PubMed
  • Domains: 5 alpha helices, 2 beta sheets, MarR-fold with wHTH motif, alpha4 major groove recognition helix, beta2 and 3 form the wing; alpha5 dimer interface PubMed
  • Modification: oxidized to Cys14-Cys49' intersubunit disulfides by disulfide stress PubMed
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasmic

Database entries

  • Structure: tba
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon: yybR (hypR)(according to DBTBS)
  • Regulation: activated by disulfide stress conditions (diamide, NaOCl) in vivo and in vitro PubMed
  • Regulatory mechanism: redox-controlled by Cys14-Cys49' intersubunit disulfide formation by diamide and NaOCl in vitro and vivo PubMed


  • Additional information: Cys14 and Cys49' are about 8-9 Angstroem apart in reduced HypR-Dimer, oxidation moves the major groove recognition alpha4 helices of the HypR dimer about 4 Angstroem towards each other that leads to activation of HypR PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Haike Antelmann,University of Greifswald, Germany

Your additional remarks

References