SigW

• Description: RNA polymerase ECF-type sigma factor SigW, activated by alkaline shock and by polymyxin B, vancomycin, cephalosporin C, D-cycloserine, and triton X-100
  
  

Gene name	sigW
Synonyms	ybbL
Essential	no
Product	RNA polymerase ECF-type sigma factor SigW
Function	resistance against SdpC that functions in detoxification and/or production of antimicrobial compounds
Interactions involving this protein in SubtInteract: SigW
MW, pI	21 kDa, 8.614
Gene length, protein length	561 bp, 187 aa
Immediate neighbours	trnSL-Gln2, ybbM
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

transcription, sigma factors and their control, cell envelope stress proteins (controlled by SigM, V, W, X, Y)

This gene is a member of the following regulons

AbrB regulon, SigW regulon

The gene

Basic information

• Locus tag: BSU01730

Phenotypes of a mutant

• sensitive to ß-lactam antibiotics such as cefuroxime and to fosfomycin PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Additional information

• A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: ECF subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity: RsiW acts as antagonist of SigW (anti-SigW)

• Interactions:
  • RsiW-SigW, SigW-RpoB-RpoC

• Localization:

Database entries

• Structure:

• UniProt: Q45585

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: sigW-rsiW PubMed

• Sigma factor: SigW PubMed

• Regulation:
  • induced under conditions of alkali shock or in the presence of the toxin SdpC (SigW) PubMed
  • repressed during logrithmic growth (AbrB) PubMed

• Regulatory mechanism:
  • AbrB: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

John Helmann, Cornell University, USA Homepage

Thomas Wiegert, University of Bayreuth, Germany Homepage

References

Additional publications: PubMed

The SigW regulon

Thorsten Mascher, Anna-Barbara Hachmann, John D Helmann
Regulatory overlap and functional redundancy among Bacillus subtilis extracytoplasmic function sigma factors.
J Bacteriol: 2007, 189(19);6919-27
[PubMed:17675383] [WorldCat.org] [DOI] (P p)

Bronwyn G Butcher, John D Helmann
Identification of Bacillus subtilis sigma-dependent genes that provide intrinsic resistance to antimicrobial compounds produced by Bacilli.
Mol Microbiol: 2006, 60(3);765-82
[PubMed:16629676] [WorldCat.org] [DOI] (P p)

Min Cao, Phil A Kobel, Maud M Morshedi, Ming Fang Winston Wu, Chris Paddon, John D Helmann
Defining the Bacillus subtilis sigma(W) regulon: a comparative analysis of promoter consensus search, run-off transcription/macroarray analysis (ROMA), and transcriptional profiling approaches.
J Mol Biol: 2002, 316(3);443-57
[PubMed:11866510] [WorldCat.org] [DOI] (P p)

M S Turner, J D Helmann
Mutations in multidrug efflux homologs, sugar isomerases, and antimicrobial biosynthesis genes differentially elevate activity of the sigma(X) and sigma(W) factors in Bacillus subtilis.
J Bacteriol: 2000, 182(18);5202-10
[PubMed:10960106] [WorldCat.org] [DOI] (P p)

X Huang, A Gaballa, M Cao, J D Helmann
Identification of target promoters for the Bacillus subtilis extracytoplasmic function sigma factor, sigma W.
Mol Microbiol: 1999, 31(1);361-71
[PubMed:9987136] [WorldCat.org] [DOI] (P p)

X Huang, K L Fredrick, J D Helmann
Promoter recognition by Bacillus subtilis sigmaW: autoregulation and partial overlap with the sigmaX regulon.
J Bacteriol: 1998, 180(15);3765-70
[PubMed:9683469] [WorldCat.org] [DOI] (P p)