AlsR

• Description: transcriptional activator of the alsS-alsD operon
  
  

Gene name	alsR
Synonyms
Essential	no
Product	transcriptional activator(LysR family)
Function	regulation of acetoin synthesis (alsS-alsD)
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	34 kDa, 7.288
Gene length, protein length	906 bp, 302 aa
Immediate neighbours	alsS, ywrK
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

carbon core metabolism, transcription factors and their control

The AlsR regulon:

• alsS-alsD

The gene

Basic information

• Locus tag: BSU36020

Phenotypes of a mutant

no acetoin production

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

Additional information

• A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: binds the promoter region of the alsS-alsD operon in the presence of acetate, activates transcription

• Protein family: LysR family of transcription factors

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: Q04778

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: AMBs3 (spc), available in the Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Elisabeth Härtig, Braunschweig, Germany Homepage

References

Claudia Frädrich, Anika March, Kerstin Fiege, Anja Hartmann, Dieter Jahn, Elisabeth Härtig
The transcription factor AlsR binds and regulates the promoter of the alsSD operon responsible for acetoin formation in Bacillus subtilis.
J Bacteriol: 2012, 194(5);1100-12
[PubMed:22178965] [WorldCat.org] [DOI] (I p)

Christopher T Brown, Laura K Fishwick, Binna M Chokshi, Marissa A Cuff, Jay M Jackson, Travis Oglesby, Alison T Rioux, Enrique Rodriguez, Gregory S Stupp, Austin H Trupp, James S Woollcombe-Clarke, Tracy N Wright, William J Zaragoza, Jennifer C Drew, Eric W Triplett, Wayne L Nicholson
Whole-genome sequencing and phenotypic analysis of Bacillus subtilis mutants following evolution under conditions of relaxed selection for sporulation.
Appl Environ Microbiol: 2011, 77(19);6867-77
[PubMed:21821766] [WorldCat.org] [DOI] (I p)

M C Renna, N Najimudin, L R Winik, S A Zahler
Regulation of the Bacillus subtilis alsS, alsD, and alsR genes involved in post-exponential-phase production of acetoin.
J Bacteriol: 1993, 175(12);3863-75
[PubMed:7685336] [WorldCat.org] [DOI] (P p)