Difference between revisions of "WalR"
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===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| − | + | * essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed] | |
| − | essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed] | + | * a constitutively active form of [[WalR]] ([[WalR]]-R204C) results in constitutive expression of the [[WalR]] regulon, and in combination with a ''[[sepF]]'' mutation to the formation of cell wall-less L-forms {{PubMed|22122227}} |
=== Database entries === | === Database entries === | ||
| Line 127: | Line 127: | ||
=Labs working on this gene/protein= | =Labs working on this gene/protein= | ||
| + | * [[Kevin Devine]], Dublin | ||
=Your additional remarks= | =Your additional remarks= | ||
| Line 136: | Line 137: | ||
<pubmed>17581128,10878122,12950927, </pubmed> | <pubmed>17581128,10878122,12950927, </pubmed> | ||
==Other original Publications== | ==Other original Publications== | ||
| − | <pubmed>18573169,17350627,10094672,17827301,9829949,16030236,19574649, 20059685 20111065 20167622 20834167 </pubmed> | + | <pubmed>18573169,17350627,10094672,17827301,9829949,16030236,19574649, 20059685 20111065 20167622 20834167 22122227 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 16:12, 30 November 2011
- Description: two-component response regulator, controls cell wall metabolism
| Gene name | walR |
| Synonyms | yycF |
| Essential | yes PubMed |
| Product | two-component response regulator |
| Function | control of cell wall metabolism |
| Interactions involving this protein in SubtInteract: WalR | |
| MW, pI | 27 kDa, 4.876 |
| Gene length, protein length | 705 bp, 235 aa |
| Immediate neighbours | walK, trnY-Phe |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
cell wall/ other, transcription factors and their control, essential genes, phosphoproteins
This gene is a member of the following regulons
The WalR regulon
The gene
Basic information
- Locus tag: BSU40410
Phenotypes of a mutant
- essential PubMed
- a constitutively active form of WalR (WalR-R204C) results in constitutive expression of the WalR regulon, and in combination with a sepF mutation to the formation of cell wall-less L-forms PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: OmpR family of two-component transcription regulators
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification: phosphorylated by WalK on an Asp residue
- Cofactor(s):
- Effectors of protein activity: phosphorylation likely affects DNA-binding activity
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- UniProt: P37478
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation: expressed during vegetative growth, repressed during stationary phase PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
- Kevin Devine, Dublin
Your additional remarks
References
Reviews
Sarah Dubrac, Paola Bisicchia, Kevin M Devine, Tarek Msadek
A matter of life and death: cell wall homeostasis and the WalKR (YycGF) essential signal transduction pathway.
Mol Microbiol: 2008, 70(6);1307-22
[PubMed:19019149]
[WorldCat.org]
[DOI]
(I p)
Sarah Dubrac, Tarek Msadek
Tearing down the wall: peptidoglycan metabolism and the WalK/WalR (YycG/YycF) essential two-component system.
Adv Exp Med Biol: 2008, 631;214-28
[PubMed:18792692]
[WorldCat.org]
[DOI]
(P p)
Malcolm E Winkler, James A Hoch
Essentiality, bypass, and targeting of the YycFG (VicRK) two-component regulatory system in gram-positive bacteria.
J Bacteriol: 2008, 190(8);2645-8
[PubMed:18245295]
[WorldCat.org]
[DOI]
(I p)
The WalR regulon
Other original Publications
