NrnA

Description: oligoribonuclease (nanoRNase), 3',5'-bisphosphate nucleotidase

Gene name	nrnA
Synonyms	ytqI
Essential	no
Product	oligoribonuclease (nanoRNase), 3',5'-bisphosphate nucleotidase
Function	degradation of RNA oligonucleotides,
MW, pI	34 kDa, 4.668
Gene length, protein length	939 bp, 313 aa
Immediate neighbours	ytrI, ytpI
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

Rnases

The gene

Basic information

• Locus tag: BSU29250

Phenotypes of a mutant

The mutant requires cysteine PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • Adenosine 3',5'-bisphosphate + H₂O = adenosine 5'-phosphate + phosphate (according to Swiss-Prot)
  • oligoribonuclease (nanoRNAse), 3',5'-bisphosphate nucleotidase PubMed
  • hydrolyze oligo(deoxy)ribonucleotides in a 5'-3' direction PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: Cytoplasm (Homogeneous) PubMed

Database entries

• Structure:

• UniProt: O34600

• KEGG entry: [3]

• E.C. number: 3.1.3.7

Expression and regulation

• Operon: nrnA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Claudiu M Nelersa, Brad J Schmier, Arun Malhotra
Purification and crystallization of Bacillus subtilis NrnA, a novel enzyme involved in nanoRNA degradation.
Acta Crystallogr Sect F Struct Biol Cryst Commun: 2011, 67(Pt 10);1235-8
[PubMed:22102036] [WorldCat.org] [DOI] (I p)

Taisuke Wakamatsu, Kwang Kim, Yuri Uemura, Noriko Nakagawa, Seiki Kuramitsu, Ryoji Masui
Role of RecJ-like protein with 5'-3' exonuclease activity in oligo(deoxy)nucleotide degradation.
J Biol Chem: 2011, 286(4);2807-16
[PubMed:21087930] [WorldCat.org] [DOI] (I p)

Ming Fang, Wencke-Maria Zeisberg, Ciaran Condon, Vasily Ogryzko, Antoine Danchin, Undine Mechold
Degradation of nanoRNA is performed by multiple redundant RNases in Bacillus subtilis.
Nucleic Acids Res: 2009, 37(15);5114-25
[PubMed:19553197] [WorldCat.org] [DOI] (I p)

Jiaqin Zhang, Indranil Biswas
3'-Phosphoadenosine-5'-phosphate phosphatase activity is required for superoxide stress tolerance in Streptococcus mutans.
J Bacteriol: 2009, 191(13);4330-40
[PubMed:19429620] [WorldCat.org] [DOI] (I p)

Undine Mechold, Gang Fang, Saravuth Ngo, Vasily Ogryzko, Antoine Danchin
YtqI from Bacillus subtilis has both oligoribonuclease and pAp-phosphatase activity.
Nucleic Acids Res: 2007, 35(13);4552-61
[PubMed:17586819] [WorldCat.org] [DOI] (I p)

Jean-Christophe Meile, Ling Juan Wu, S Dusko Ehrlich, Jeff Errington, Philippe Noirot
Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory.
Proteomics: 2006, 6(7);2135-46
[PubMed:16479537] [WorldCat.org] [DOI] (P p)

Alia Lapidus, Nathalie Galleron, Alexei Sorokin, S Dusko Ehrlich
Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region.
Microbiology (Reading): 1997, 143 ( Pt 11);3431-3441
[PubMed:9387221] [WorldCat.org] [DOI] (P p)