RapC

• Description: response regulator aspartate phosphatase, controls ComA activity
  
  

Gene name	rapC
Synonyms	yclL
Essential	no
Product	response regulator aspartate phosphatase
Function	control of ComA activity
Interactions involving this protein in SubtInteract: RapC
MW, pI	45 kDa, 5.024
Gene length, protein length	1146 bp, 382 aa
Immediate neighbours	yclK, phrC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

genetic competence, protein modification, transcription factors and their control, quorum sensing

This gene is a member of the following regulons

CodY regulon, ComA regulon

The gene

Basic information

• Locus tag: BSU03770

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: control of ComA activity PubMed

• Protein family: RAP family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity: binding of PhrC to RapC prevents it from binding ComA PubMed

• Interactions:
  • RapC-PhrC PubMed, RapC-ComA PubMed

• Localization:

Database entries

• Structure:

• UniProt: P94415

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: rapC-phrC PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • expressed at high cell density (ComA) PubMed

• Regulatory mechanism:
  • ComA: transcription activation (ComA) PubMed
  • CodY: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Natalia Comella, Alan D Grossman
Conservation of genes and processes controlled by the quorum response in bacteria: characterization of genes controlled by the quorum-sensing transcription factor ComA in Bacillus subtilis.
Mol Microbiol: 2005, 57(4);1159-74
[PubMed:16091051] [WorldCat.org] [DOI] (P p)

Leighton Core, Marta Perego
TPR-mediated interaction of RapC with ComA inhibits response regulator-DNA binding for competence development in Bacillus subtilis.
Mol Microbiol: 2003, 49(6);1509-22
[PubMed:12950917] [WorldCat.org] [DOI] (P p)

B A Lazazzera, I G Kurtser, R S McQuade, A D Grossman
An autoregulatory circuit affecting peptide signaling in Bacillus subtilis.
J Bacteriol: 1999, 181(17);5193-200
[PubMed:10464187] [WorldCat.org] [DOI] (P p)