Difference between revisions of "PGP888"
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<big>'''A Novel Factor Controlling Bistability in ''Bacillus subtilis'': The YmdB Protein Affects</big> | <big>'''A Novel Factor Controlling Bistability in ''Bacillus subtilis'': The YmdB Protein Affects</big> | ||
<big>Flagellin Expression and Biofilm Formation.''' </big> | <big>Flagellin Expression and Biofilm Formation.''' </big> | ||
| − | J Bacteriol. 2011 | + | <big>J Bacteriol.: 2011, 193(21):5997-6007. </big> |
[http://www.ncbi.nlm.nih.gov/pubmed/21856853 PubMed:21856853] | [http://www.ncbi.nlm.nih.gov/pubmed/21856853 PubMed:21856853] | ||
Revision as of 15:40, 31 October 2011
The vector was constructed in the Stülke lab and it is suitable for the xylose-inducible expression of proteins in B. subtilis. The plasmid confers resistance to ampicillin and kanamycin in E. coli and B. subtilis, respectively. To transform B. subtilis, the plasmid has to be linearized with ScaI or NotI
Hints for usage
- An additional base has to be inserted in the forward primer to restore the frame.
- Don't forget a stop codon in the reverse primer.
The reference
Diethmaier C, Pietack N, Gunka K, Wrede C, Lehnik-Habrink M, Herzberg C, Hübner S, Stülke J A Novel Factor Controlling Bistability in Bacillus subtilis: The YmdB Protein Affects Flagellin Expression and Biofilm Formation. J Bacteriol.: 2011, 193(21):5997-6007. PubMed:21856853
