Difference between revisions of "YmdB"
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* '''Description:''' putative phosphatase/ phosphodiesterase <br/><br/> | * '''Description:''' putative phosphatase/ phosphodiesterase <br/><br/> | ||
| − | + | , controls bistable gene expression | |
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|style="background:#ABCDEF;" align="center"| '''Product''' || putative phosphatase/ phosphodiesterase | |style="background:#ABCDEF;" align="center"| '''Product''' || putative phosphatase/ phosphodiesterase | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"|'''Function''' || | + | |style="background:#ABCDEF;" align="center"|'''Function''' || control of bistable gene expression |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29,1 kDa, 6.50 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29,1 kDa, 6.50 | ||
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===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| − | strong overexpression of [[ | + | * strong overexpression of ''[[hag]]'' {{PubMed|21856853}} |
| + | * defective in [[biofilm formation]] {{PubMed|21856853}} | ||
=== Database entries === | === Database entries === | ||
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=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' ''[[rny]]-[[ymdB]]'' | + | * '''Operon:''' ''[[rny]]-[[ymdB]]'' (weak readthrough transcription) {{PubMed|21856853}} |
* '''[[Sigma factor]]:''' | * '''[[Sigma factor]]:''' | ||
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* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| − | * '''Additional information:''' there is a terminator between ''rny'' and ''[[ymdB]]'', most transcripts terminate there | + | * '''Additional information:''' there is a terminator between ''rny'' and ''[[ymdB]]'', most transcripts terminate there {{PubMed|21856853}} |
=Biological materials = | =Biological materials = | ||
Revision as of 11:20, 23 August 2011
- Description: putative phosphatase/ phosphodiesterase
, controls bistable gene expression
| Gene name | ymdB |
| Synonyms | |
| Essential | no |
| Product | putative phosphatase/ phosphodiesterase |
| Function | control of bistable gene expression |
| MW, pI | 29,1 kDa, 6.50 |
| Gene length, protein length | 792 bp, 264 amino acids |
| Immediate neighbours | rny, spoVS |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU16970
Phenotypes of a mutant
- strong overexpression of hag PubMed
- defective in biofilm formation PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: phosphatase activity toward phosphoenolpyruvate and phosphodiesterase activity toward 2',3'-cAMP (the Deinococcus ortholog) PubMed
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- UniProt: O31775
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Regulation: constitutive
- Regulatory mechanism:
- Additional information: there is a terminator between rny and ymdB, most transcripts terminate there PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
- Antibody:
Labs working on this gene/protein
Jörg Stülke, University of Göttingen, Germany Homepage
Your additional remarks
References
The most recent publications on B. subtilis
Diethmaier C, Pietack N, Gunka K, Wrede C, Lehnik-Habrink M, Herzberg C, Hübner S, Stülke J A Novel Factor Controlling Bistability in Bacillus subtilis: The YmdB Protein Affects Flagellin Expression and Biofilm Formation. J Bacteriol. 2011 Aug 19. [Epub ahead of print] PubMed:21856853
Functional and structural analysis of orthologs in other organisms
