Cmk

• Description: cytidylate kinase (CMP, dCMP)
  
  

Gene name	cmk
Synonyms	jofC, ypfC
Essential	yes PubMed
Product	cytidylate kinase (CMP, dCMP)
Function	synthesis of CTP and dCTP
Metabolic function and regulation of this protein in SubtiPathways: Pyrimidines, Nucleotides (regulation)
MW, pI	24 kDa, 4.999
Gene length, protein length	672 bp, 224 aa
Immediate neighbours	ypfD, ypfB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides, essential genes

The gene

Basic information

• Locus tag: BSU22890

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + (d)CMP = ADP + (d)CDP (according to Swiss-Prot)

• Protein family: Type 1 subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1CKE (from Escherichia coli, 38% identity, 54% similarity) PubMed

• UniProt: P38493

• KEGG entry: [2]

• E.C. number: 2.7.4.14

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

C P Schultz, L Ylisastigui-Pons, L Serina, H Sakamoto, H H Mantsch, J Neuhard, O Bârzu, A M Gilles
Structural and catalytic properties of CMP kinase from Bacillus subtilis: a comparative analysis with the homologous enzyme from Escherichia coli.
Arch Biochem Biophys: 1997, 340(1);144-53
[PubMed:9126287] [WorldCat.org] [DOI] (P p)