MreB

• Description: cell shape-determining protein, forms filaments, the polymers control/restrict the mobility of the cell wall elongation enzyme complex
  
  

Gene name	mreB
Synonyms	divIVB
Essential	yes PubMed
Product	cell shape-determining protein
Function	cell shape determination
MW, pI	35 kDa, 4.901
Gene length, protein length	1011 bp, 337 aa
Immediate neighbours	mreC, radC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

cell shape, cell envelope stress proteins (controlled by SigM, W, X, Y), essential genes, membrane proteins

This gene is a member of the following regulons

SigM regulon

The gene

Basic information

• Locus tag: BSU28030

Phenotypes of a mutant

• essential PubMed
• the mutation can be suppressed by inactivation of ponA, ptsI, ccpA PubMed, by overexpression of YvcK PubMed, or by addition of 5 mM magnesium to the growth medium PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • forms straight filaments in a heterologous system PubMed
  • polymerizes in the presence of millimolar divalent cations, binds and hydrolyzes GTP and ATP PubMed

• Protein family: ftsA/mreB family (according to Swiss-Prot)

• Paralogous protein(s): Mbl, MreBH

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • part of the cell wall biosynthetic complex PubMed
  • MreB-TufA PubMed
  • MreB-Mbl PubMed
  • MreB-MreBH PubMed

• Localization:
  • during logarithmic growth, MreB forms discrete patches thst move processively along peripheral tracks perpendicular to the cell axis PubMed
  • forms transverse bands as cells enter the stationary phase PubMed
  • close to the inner surface of the cytoplasmic membrane PubMed
  • reports on helical structures formed by MreB PubMed seem to be misinterpretation of data PubMed

Database entries

• Structure: 1JCE (from Thermotoga maritima) PubMed

• UniProt: Q01465

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • radC-mreB-mreC-mreD-minC-minD PubMed
  • mreB-mreC-mreD-minC-minD PubMed

• Sigma factor:
  • radC: SigM PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in the labs of Jeff Errington and Boris Görke

• Antibody: available in the Jeff Errington and Peter Graumann labs

Labs working on this gene/protein

Jeff Errington, Newcastle University, UK homepage

Peter Graumann, Freiburg University, Germany homepage

References

Reviews

Additional reviews: PubMed