SubtInteract

Protein-protein interactions are essential for many activities of any living cell. These interactions involve multi-protein complexes that take part in central processes such as DNA replication, transcription or translation. Protein-protein interactions may also be involved in a variety of regulatory events. Metabolic enzymes do often form transien complexes that represent a complete pathways. These complexes are called metabolon. Finally, many interactions may be of a transient nature.

Methods to detect protein-protein interactions

• Bacterial Two Hybrid System: BACTH PubMed

• Yeast Two Hybrid System PubMed

• SPINE: A method to detect in vivo protein-protein interactions after cross-linking PubMed

• TAP-Tag purification PubMed

• in vivo detection of protein-protein interaction using DivIVA and GFP PubMed

Attention: Each technique detects only about 33% of all interactions PubMed