Difference between revisions of "SubtInteract"

From SubtiWiki
Jump to: navigation, search
(Methods to detect protein-protein interactions)
Line 12: Line 12:
 
* ''in vivo'' detection of protein-protein interaction using [[DivIVA]] and GFP {{PubMed|19698693}}
 
* ''in vivo'' detection of protein-protein interaction using [[DivIVA]] and GFP {{PubMed|19698693}}
  
'''Attention:''' Each technique detects only about 33% of all interactions {{PubMed|10504710,11403571}}
+
'''Attention:''' Each technique detects only about 33% of all interactions {{PubMed|20805792,19060903}}
  
 
==Important publications==
 
==Important publications==
<pubmed>20658969 18228443 18219467 17953394 15264234 14706816 12127457 11827824 11377797 11306254 10851163 10842303  </pubmed>
+
<pubmed>20658969 18228443 18219467 17953394 15264234 14706816 12127457 11827824 11377797 11306254 10851163 10842303 18366733 </pubmed>

Revision as of 21:21, 3 January 2011

Protein-protein interactions are essential for many activities of any living cell. These interactions involve multi-protein complexes that take part in central processes such as DNA replication, transcription or translation. Protein-protein interactions may also be involved in a variety of regulatory events. Finally, many interactions may be of a transient nature.

Methods to detect protein-protein interactions

  • Yeast Two Hybrid System PubMed
  • SPINE: A method to detect in vivo protein-protein interactions after cross-linking PubMed
  • in vivo detection of protein-protein interaction using DivIVA and GFP PubMed

Attention: Each technique detects only about 33% of all interactions PubMed

Important publications