LevB

• Description: endolevanase
  
  

Gene name	levB
Synonyms	yveB
Essential	no
Product	endolevanase
Function	levan degradation
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	58 kDa, 4.993
Gene length, protein length	1548 bp, 516 aa
Immediate neighbours	sacB, yveA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

utilization of specific carbon sources, membrane proteins

This gene is a member of the following regulons

DegU regulon, SacY regulon

The gene

Basic information

• Locus tag: BSU34460

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Hydrolysis of (2->6)-beta-D-fructofuranan, to remove successive disaccharide residues as levanbiose, i (according to Swiss-Prot)

• Protein family: glycosyl hydrolase 32 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O07003

• KEGG entry: [3]

• E.C. number: 3.2.1.64

Expression and regulation

• Operon: sacB-levB-yveA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induction by sucrose (at high concentration) (SacY) PubMed

• Regulatory mechanism:
  • SacY: binding to a RNA switch (transcriptional antitermination) PubMed
  • DegU-P: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Y Pereira, M F Petit-Glatron, R Chambert
yveB, Encoding endolevanase LevB, is part of the sacB-yveB-yveA levansucrase tricistronic operon in Bacillus subtilis.
Microbiology (Reading): 2001, 147(Pt 12);3413-9
[PubMed:11739774] [WorldCat.org] [DOI] (P p)

S Aymerich, M Steinmetz
Cloning and preliminary characterization of the sacS locus from Bacillus subtilis which controls the regulation of the exoenzyme levansucrase.
Mol Gen Genet: 1987, 208(1-2);114-20
[PubMed:3039303] [WorldCat.org] [DOI] (P p)

H Shimotsu, D J Henner
Modulation of Bacillus subtilis levansucrase gene expression by sucrose and regulation of the steady-state mRNA level by sacU and sacQ genes.
J Bacteriol: 1986, 168(1);380-8
[PubMed:2428811] [WorldCat.org] [DOI] (P p)