Difference between revisions of "Rnc"

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|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''rncS ''
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''rncS ''
 
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|style="background:#ABCDEF;" align="center"| '''Essential''' ||  
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|style="background:#ABCDEF;" align="center"| '''Essential''' || yes
 
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|-
 
|style="background:#ABCDEF;" align="center"| '''Product''' || endoribonuclease III  
 
|style="background:#ABCDEF;" align="center"| '''Product''' || endoribonuclease III  
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 +
 +
''rnc'' is essential [http://www.ncbi.nlm.nih.gov/sites/entrez/11123676 PubMed]
  
 
=== Database entries ===
 
=== Database entries ===

Revision as of 09:01, 9 February 2009

  • Description: RNase III

Gene name rnc
Synonyms rncS
Essential yes
Product endoribonuclease III
Function cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor
MW, pI 28 kDa, 8.076
Gene length, protein length 747 bp, 249 aa
Immediate neighbours
Gene sequence (+200bp) Protein sequence
Genetic context
Rnc context.gif



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

rnc is essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry:
  • E.C. number: 3.1.26.3

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • Antibody:

Labs working on this gene/protein

David Bechhofer, Mount Sinai School, New York, USA Homepage

Your additional remarks

References

  1. Wang, W. & Bechhofer, D. H. (1997). Bacillus subtilis RNase III gene: cloning, function of the gene in Escherichia coli, and construction of Bacillus subtilis strains with altered rnc loci. J Bacteriol 179, 7379-7385. PubMed
  2. Herskovitz, M. A. & Bechhofer, D. H. (2000). Endoribonuclease RNase III is essential in Bacillus subtilis. Mol Microbiol 38:1027-1033. PubMed
  3. Mitra, S. & Bechhofer, D. H. (1994). Substrate specificity of an RNase III-like activity from Bacillus subtilis. J Biol Chem 269:31450-31456. PubMed
  4. Oguro, A., Kakeshita, H., Nakamura, K., Yamane, K., Wang, W. & Bechhofer, D. H. (1998). Bacillus subtilis RNase III cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor. J Biol Chem 273:19542-19547. PubMed
  5. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed