Difference between revisions of "SucD"
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<br/><br/><br/><br/><br/><br/> | <br/><br/><br/><br/><br/><br/> | ||
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| + | = [[Categories]] containing this gene/protein = | ||
| + | {{SubtiWiki category|[[ATP synthesis]]}}, | ||
| + | {{SubtiWiki category|[[carbon core metabolism]]}}, | ||
| + | {{SubtiWiki category|[[phosphoproteins]]}} | ||
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| + | = This gene is a member of the following [[regulons]] = | ||
| + | {{SubtiWiki regulon|[[CcpA regulon]]}} | ||
=The gene= | =The gene= | ||
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=The protein= | =The protein= | ||
Revision as of 19:37, 8 December 2010
- Description: succinyl-CoA synthetase (alpha subunit)
| Gene name | sucD |
| Synonyms | |
| Essential | no |
| Product | succinyl-CoA synthetase (alpha subunit) |
| Function | TCA cycle |
| Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism | |
| MW, pI | 31 kDa, 5.587 |
| Gene length, protein length | 900 bp, 300 aa |
| Immediate neighbours | sucC, dprA |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
Categories containing this gene/protein
ATP synthesis, carbon core metabolism, phosphoproteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU16100
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + succinate + CoA = ADP + phosphate + succinyl-CoA (according to Swiss-Prot)
- Protein family: succinate/malate CoA ligase alpha subunit family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information: Reversible Michaelis-Menten FEBS Letters
- Domains:
- Modification: phosphorylation on (Ser-19 OR Thr-20) PubMed
- Cofactor(s):
- Effectors of protein activity:
- Inhibited by 2-oxoglutarate, ATP and NADH FEBS Letters
- GTP is not accepted by the enzyme FEBS Letters
- Localization:
Database entries
- Structure: 1JKJ (E. coli)
- UniProt: P80865
- KEGG entry: [3]
- E.C. number: 6.2.1.5
Additional information
The enzyme is a dimer FEBS Letters
Expression and regulation
- Sigma factor:
- Additional information:
Biological materials
- Mutant: GP720 (spc), available in Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
