YitB

• Description: phospho-adenylylsulfate sulfotransferase
  
  

Gene name	yitB
Synonyms
Essential	no
Product	phospho-adenylylsulfate sulfotransferase
Function	sulfate reduction and activation
Metabolic function and regulation of this protein in SubtiPathways: Cys, Met & Sulfate assimilation
MW, pI	27 kDa, 5.77
Gene length, protein length	708 bp, 236 aa
Immediate neighbours	yitA, yitC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU10930

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

sulfur metabolism, sporulation proteins

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin (according to Swiss-Prot)

• Protein family: CysH subfamily (according to Swiss-Prot)

• Paralogous protein(s): CysH

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O06737

• KEGG entry: [3]

• E.C. number: 1.8.4.8

Expression and regulation

• Operon: yitB-yitA-yisZ (according to DBTBS)

• Sigma factor: SigK PubMed

• Regulation:
  • expressed during sporulation in the mother cell (SigK) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836] [WorldCat.org] [DOI] (I p)