SrfAD

• Description: surfactin synthetase / competence
  
  

Gene name	srfAD
Synonyms	comL
Essential	no
Product	surfactin synthetase / competence
Function	antibiotic synthesis
MW, pI	27 kDa, 5.22
Gene length, protein length	726 bp, 242 aa
Immediate neighbours	srfAC, ycxA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU03520

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

miscellaneous metabolic pathways, biosynthesis of antibacterial compounds

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: thioesterase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 2RON, 2K2Q

• UniProt: Q08788

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: srfAA-srfAB-comS-srfAC-srfAD PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • repressed during hydrogen peroxide stress (PerR) PubMed
  • expressed at high cell density (ComA) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed
  • ComA: transcription activation PubMed
  • PerR: transcription activation PubMed
  • Spx: transcription repression PubMed
  • Abh: transcription repressionPubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Massimiliano Marvasi, Pieter T Visscher, Lilliam Casillas Martinez
Exopolymeric substances (EPS) from Bacillus subtilis: polymers and genes encoding their synthesis.
FEMS Microbiol Lett: 2010, 313(1);1-9
[PubMed:20735481] [WorldCat.org] [DOI] (I p)