SrfAB

• Description: surfactin synthetase / competence
  
  

Gene name	srfAB
Synonyms	comL
Essential	no
Product	surfactin synthetase / competence
Function	antibiotic synthesis
MW, pI	400 kDa, 4.903
Gene length, protein length	10761 bp, 3587 aa
Immediate neighbours	srfAA, comS
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU03490

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

miscellaneous metabolic pathways, biosynthesis of antibacterial compounds, membrane proteins, phosphoproteins

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on Ser-999 AND Ser-2045 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: membrane PubMed

Database entries

• Structure:

• UniProt: Q04747

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: srfAA-srfAB-comS-srfAC-srfAD PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • repressed during hydrogen peroxide stress (PerR) PubMed
  • expressed at high cell density (ComA) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed
  • ComA: transcription activation PubMed
  • PerR: transcription activation PubMed
  • Spx: transcription repression PubMed
  • Abh: transcription repressionPubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: