PucH

• Description: allantoinase
  
  

Gene name	pucH
Synonyms	yunH
Essential	no
Product	allantoinase
Function	purine utilization
Metabolic function and regulation of this protein in SubtiPathways: Purine catabolism, Nucleotides (regulation)
MW, pI	48 kDa, 5.062
Gene length, protein length	1338 bp, 446 aa
Immediate neighbours	yunG, pucR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU32410

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

utilization of nucleotides

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: (S)-allantoin + H₂O = allantoate (according to Swiss-Prot)

• Protein family: Allantoinase subfamily (according to Swiss-Prot)

• Paralogous protein(s): PyrC

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3HM7 (from Bacillus halodurans, 44% identity, 65% similarity)

• UniProt: O32137

• KEGG entry: [3]

• E.C. number: 3.5.2.5

Expression and regulation

• Operon: pucH PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced in the presence of purine nucleotides (inducer: allantoin) (PucR) PubMed

• Regulatory mechanism:
  • PucR: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Lars Beier, Per Nygaard, Hanne Jarmer, Hans H Saxild
Transcription analysis of the Bacillus subtilis PucR regulon and identification of a cis-acting sequence required for PucR-regulated expression of genes involved in purine catabolism.
J Bacteriol: 2002, 184(12);3232-41
[PubMed:12029039] [WorldCat.org] [DOI] (P p)

A C Schultz, P Nygaard, H H Saxild
Functional analysis of 14 genes that constitute the purine catabolic pathway in Bacillus subtilis and evidence for a novel regulon controlled by the PucR transcription activator.
J Bacteriol: 2001, 183(11);3293-302
[PubMed:11344136] [WorldCat.org] [DOI] (P p)