PatB

• Description: cystathione-beta-lyase
  
  

Gene name	patB
Synonyms
Essential	no
Product	cystathione-beta-lyase
Function	biosynthesis of methionine
Metabolic function and regulation of this protein in SubtiPathways: Cys, Met & Sulfate assimilation
MW, pI	42 kDa, 5.439
Gene length, protein length	1161 bp, 387 aa
Immediate neighbours	yugE, kinB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU31440

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-cystathionine + H₂O = L-homocysteine + NH₃ + pyruvate (according to Swiss-Prot)

• Protein family: MalY/patB cystathionine beta-lyase subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: Q08432

• KEGG entry: [3]

• E.C. number: 4.4.1.8

Expression and regulation

• Operon: patB PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

S Auger, M P Gomez, A Danchin, I Martin-Verstraete
The PatB protein of Bacillus subtilis is a C-S-lyase.
Biochimie: 2005, 87(2);231-8
[PubMed:15760717] [WorldCat.org] [DOI] (P p)

Bauke Oudega, Gregory Koningstein, Luísa Rodrigues, Maria de Sales Ramon, Helmut Hilbert, Andreas Düsterhöft, Thomas M Pohl, Thomas Weitzenegger
Analysis of the Bacillus subtilis genome: cloning and nucleotide sequence of a 62 kb region between 275 degrees (rrnB) and 284 degrees (pai).
Microbiology (Reading): 1997, 143 ( Pt 8);2769-2774
[PubMed:9274030] [WorldCat.org] [DOI] (P p)